3pvh

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[[Image:3pvh.png|left|200px]]
 
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==Structural and Functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3==
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The line below this paragraph, containing "STRUCTURE_3pvh", creates the "Structure Box" on the page.
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<StructureSection load='3pvh' size='340' side='right'caption='[[3pvh]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3pvh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PVH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PVH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3pvh| PDB=3pvh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pvh OCA], [https://pdbe.org/3pvh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pvh RCSB], [https://www.ebi.ac.uk/pdbsum/3pvh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pvh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/U603_ARATH U603_ARATH]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The membrane protein AtTLP18.3 contains a domain of unknown function, DUF477; it forms a polysome with photosynthetic apparatuses in the thylakoid lumen. To explore the molecular function of AtTLP18.3, we resolved its crystal structures with residues 83 to 260, the DUF477 only, and performed a series of biochemical analyses to discover its function. The gene expression of AtTLP18.3 followed a circadian rhythm. X-ray crystallography revealed the folding of AtTLP18.3 as a three-layer sandwich with 3 alpha-helices in the upper layer, 4 beta-sheets in the middle layer, and 2 alpha-helices in the lower layer, which resembles a Rossmann fold. Structural comparison suggested that AtTLP18.3 might be a phosphatase. The enzymatic activity of AtTLP18.3 was further confirmed by phosphatase assay with various substrates (e.g., p-nitrophenyl phosphate, 6,8 difluoro-4-methylumbelliferyl phosphate, O-phospho-L-Serine [pSer] and several synthetic phosphopeptides). Furthermore, we obtained the structure of AtTLP18.3 in complex with pSer to identify the binding site of AtTLP18.3. Our structural and biochemical studies revealed that AtTLP18.3 has a molecular function of a novel acid phosphatase in the thylakoid lumen. DUF477 is accordingly renamed the thylakoid acid phosphatase (TAP) domain.
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===Structural and Functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3===
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Structural and Functional Assays of AtTLP18.3 Identify Its Novel Acid Phosphatase Activity in Thylakoid Lumen.,Wu HY, Liu MS, Lin TP, Cheng YS Plant Physiol. 2011 Sep 9. PMID:21908686<ref>PMID:21908686</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pvh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21908686 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21908686}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3pvh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PVH OCA].
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==Reference==
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<ref group="xtra">PMID:021908686</ref><references group="xtra"/>
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Cheng, Y S.]]
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[[Category: Large Structures]]
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[[Category: Lin, T P.]]
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[[Category: Cheng YS]]
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[[Category: Liu, M S.]]
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[[Category: Lin TP]]
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[[Category: Wu, H Y.]]
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[[Category: Liu MS]]
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[[Category: Acid phosphatase]]
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[[Category: Wu HY]]
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[[Category: Arabidopsis thaliana thylakoid lumen]]
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[[Category: Hydrolase]]
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[[Category: Rossman fold]]
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[[Category: Tap domain]]
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Current revision

Structural and Functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3

PDB ID 3pvh

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