1lgy

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[[Image:1lgy.jpg|left|200px]]<br /><applet load="1lgy" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lgy, resolution 2.2&Aring;" />
 
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'''LIPASE II FROM RHIZOPUS NIVEUS'''<br />
 
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==Overview==
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==LIPASE II FROM RHIZOPUS NIVEUS==
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The crystal and molecular structure of Lipase II from Rhizopus niveus was, analyzed using X-ray single crystal diffraction data at a resolution of, 2.2 A. The structure was refined to an R-factor of 0.19 for all available, data. This lipase was purified and crystallized as Lipase I, which, contains two polypeptide chains combined through non-covalent interaction., However, during crystal growth, Lipase I was converted to Lipase II, which, consists of a single polypeptide chain of 269 amino acid residues, by, limited proteolysis. The structure of Lipase II shows a typical alpha/beta, hydrolase fold containing the so-called nucleophilic elbow. The catalytic, center of this enzyme is analogous to those of other neutral lipases and, serine proteases. This catalytic center is sheltered by an alpha-helix, lid, which appears in neutral lipases, opening the active site at the, oil-water interface.
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<StructureSection load='1lgy' size='340' side='right'caption='[[1lgy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lgy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizopus_niveus Rhizopus niveus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LGY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgy OCA], [https://pdbe.org/1lgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lgy RCSB], [https://www.ebi.ac.uk/pdbsum/1lgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lgy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LIP_RHINI LIP_RHINI] Hydrolyzes ester bonds of triglycerides as well as of their derived partial glycerides with a strong 1,3-positional specificity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lg/1lgy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lgy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LGY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizopus_niveus Rhizopus niveus]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic+Site'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGY OCA].
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*[[Lipase 3D Structures|Lipase 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of lipase II from Rhizopus niveus at 2.2 A resolution., Kohno M, Funatsu J, Mikami B, Kugimiya W, Matsuo T, Morita Y, J Biochem (Tokyo). 1996 Sep;120(3):505-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8902613 8902613]
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[[Category: Large Structures]]
[[Category: Rhizopus niveus]]
[[Category: Rhizopus niveus]]
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[[Category: Single protein]]
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[[Category: Funatsu J]]
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[[Category: Triacylglycerol lipase]]
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[[Category: Kohno M]]
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[[Category: Funatsu, J.]]
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[[Category: Kugimiya W]]
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[[Category: Kohno, M.]]
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[[Category: Matsuo T]]
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[[Category: Kugimiya, W.]]
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[[Category: Mikami B]]
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[[Category: Matsuo, T.]]
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[[Category: Morita Y]]
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[[Category: Mikami, B.]]
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[[Category: Morita, Y.]]
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[[Category: hydrolase (carboxylic ester)]]
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[[Category: lipase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:53:17 2008''
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Current revision

LIPASE II FROM RHIZOPUS NIVEUS

PDB ID 1lgy

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