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1akt

From Proteopedia

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[[Image:1akt.gif|left|200px]]<br />
 
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<applet load="1akt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1akt, resolution 1.8&Aring;" />
 
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'''G61N OXIDIZED FLAVODOXIN MUTANT'''<br />
 
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==Overview==
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==G61N OXIDIZED FLAVODOXIN MUTANT==
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Mutants of the electron-transfer protein flavodoxin from Desulfovibrio, vulgaris were made by site-directed mutagenesis to investigate the role of, glycine-61 in stabilizing the semiquinone of FMN by the protein and in, controlling the flavin redox potentials. The spectroscopic properties, oxidation-reduction potentials, and flavin-binding properties of the, mutant proteins, G61A/N/V and L, were compared with those of wild-type, flavodoxin. The affinities of all of the mutant apoproteins for FMN and, riboflavin were less than that of the wild-type apoprotein, and the redox, potentials of the two 1-electron steps in the reduction of the complex, with FMN were also affected by the mutations. Values for the dissociation, constants of the complexes of the apoprotein with the semiquinone and, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9622492 (full description)]]
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<StructureSection load='1akt' size='340' side='right'caption='[[1akt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1akt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akt OCA], [https://pdbe.org/1akt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akt RCSB], [https://www.ebi.ac.uk/pdbsum/1akt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH] Low-potential electron donor to a number of redox enzymes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AKT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]] with FMN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: FMN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AKT OCA]].
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*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Modulation of the redox potentials of FMN in Desulfovibrio vulgaris flavodoxin: thermodynamic properties and crystal structures of glycine-61 mutants., O'Farrell PA, Walsh MA, McCarthy AA, Higgins TM, Voordouw G, Mayhew SG, Biochemistry. 1998 Jun 9;37(23):8405-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9622492 9622492]
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[[Category: Desulfovibrio vulgaris str. Hildenborough]]
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[[Category: Desulfovibrio vulgaris]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Higgins T]]
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[[Category: Higgins, T.]]
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[[Category: Mccarthy A]]
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[[Category: Mccarthy, A.]]
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[[Category: Walsh M]]
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[[Category: Walsh, M.]]
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[[Category: FMN]]
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[[Category: electron transfer]]
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[[Category: electron transport]]
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[[Category: flavodoxin]]
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[[Category: flavoprotein]]
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[[Category: fmn]]
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[[Category: mutant]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:33:45 2007''
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Current revision

G61N OXIDIZED FLAVODOXIN MUTANT

PDB ID 1akt

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