3tho

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[[Image:3tho.png|left|200px]]
 
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==Crystal structure of Mre11:Rad50 in its ATP/ADP bound state==
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The line below this paragraph, containing "STRUCTURE_3tho", creates the "Structure Box" on the page.
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<StructureSection load='3tho' size='340' side='right'caption='[[3tho]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3tho]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3THO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3THO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6081&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3tho| PDB=3tho | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tho OCA], [https://pdbe.org/3tho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tho RCSB], [https://www.ebi.ac.uk/pdbsum/3tho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tho ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAD50_THEMA RAD50_THEMA] Involved in DNA double-strand break repair (DSBR). The Rad50/Mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of Mre11 by unwinding and/or repositioning DNA ends into the Mre11 active site (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA double-strand breaks (DSBs) threaten genome stability in all kingdoms of life and are linked to cancerogenic chromosome aberrations in humans. The Mre11:Rad50 (MR) complex is an evolutionarily conserved complex of two Rad50 ATPases and a dimer of the Mre11 nuclease that senses and processes DSBs and tethers DNA for repair. ATP binding and hydrolysis by Rad50 is functionally coupled to DNA-binding and tethering, but also regulates Mre11's nuclease in processing DNA ends. To understand how ATP controls the interaction between Mre11 and Rad50, we determined the crystal structure of Thermotoga maritima (Tm) MR trapped in an ATP/ADP state. ATP binding to Rad50 induces a large structural change from an open form with accessible Mre11 nuclease sites into a closed form. Remarkably, the NBD dimer binds in the Mre11 DNA-binding cleft blocking Mre11's dsDNA-binding sites. An accompanying large swivel of the Rad50 coiled coil domains appears to prepare the coiled coils for DNA tethering. DNA-binding studies show that within the complex, Rad50 likely forms a dsDNA-binding site in response to ATP, while the Mre11 nuclease module retains a ssDNA-binding site. Our results suggest a possible mechanism for ATP-dependent DNA tethering and DSB processing by MR.
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===Crystal structure of Mre11:Rad50 in its ATP/ADP bound state===
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ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complex.,Mockel C, Lammens K, Schele A, Hopfner KP Nucleic Acids Res. 2011 Sep 21. PMID:21937514<ref>PMID:21937514</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21937514}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3tho" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21937514 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21937514}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3tho]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3THO OCA].
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==Reference==
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<ref group="xtra">PMID:021937514</ref><references group="xtra"/>
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[[Category: Thermotoga maritima]]
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[[Category: Lammens, K.]]
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[[Category: Moeckel, C.]]
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[[Category: Abc atpase]]
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[[Category: Adenosine triphosphate]]
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[[Category: Bacterial protein]]
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[[Category: Dna break]]
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[[Category: Dna repair]]
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[[Category: Dna repair enzyme]]
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[[Category: Dna-binding protein]]
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[[Category: Double-stranded]]
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[[Category: Endodeoxyribonuclease]]
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[[Category: Exodeoxyribonuclease]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase-dna binding protein complex]]
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[[Category: Model]]
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[[Category: Molecular]]
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[[Category: Nuclease]]
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[[Category: Scattering]]
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[[Category: Small angle]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Lammens K]]
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[[Category: Moeckel C]]

Current revision

Crystal structure of Mre11:Rad50 in its ATP/ADP bound state

PDB ID 3tho

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