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1odz
From Proteopedia
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| - | [[Image:1odz.gif|left|200px]]<br /><applet load="1odz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1odz, resolution 1.40Å" /> | ||
| - | '''EXPANSION OF THE GLYCOSYNTHASE REPERTOIRE TO PRODUCE DEFINED MANNO-OLIGOSACCHARIDES'''<br /> | ||
| - | == | + | ==Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides== |
| - | Mutant endo-mannanases, in which the catalytic nucleophile has been | + | <StructureSection load='1odz' size='340' side='right'caption='[[1odz]], [[Resolution|resolution]] 1.40Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1odz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ODZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odz OCA], [https://pdbe.org/1odz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1odz RCSB], [https://www.ebi.ac.uk/pdbsum/1odz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1odz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/od/1odz_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1odz ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths. | ||
| - | + | Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.,Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG Chem Commun (Camb). 2003 Jun 21;(12):1327-9. PMID:12841226<ref>PMID:12841226</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1odz" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Mannosidase 3D structures|Mannosidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cellvibrio japonicus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Davies GJ]] | ||
| + | [[Category: Ducros VMA]] | ||
| + | [[Category: Gilbert HJ]] | ||
| + | [[Category: Jahn M]] | ||
| + | [[Category: Singh P]] | ||
| + | [[Category: Stoll D]] | ||
| + | [[Category: Szabo L]] | ||
| + | [[Category: Warren RAJ]] | ||
| + | [[Category: Withers SG]] | ||
Current revision
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides
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Categories: Cellvibrio japonicus | Large Structures | Davies GJ | Ducros VMA | Gilbert HJ | Jahn M | Singh P | Stoll D | Szabo L | Warren RAJ | Withers SG
