1of5

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[[Image:1of5.gif|left|200px]]<br /><applet load="1of5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1of5, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MEX67-MTR2'''<br />
 
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==Overview==
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==Crystal structure of Mex67-Mtr2==
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The association between Mtr2 and Mex67 is essential for the nuclear export, of bulk messenger RNA in yeast. In metazoans, the analogous function is, carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly, conserved proteins, their binding partners, Mtr2 and p15, share no, sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with, the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like, family and interacts with Mex67, forming a complex with a similar, structural architecture to that of TAP-p15. Mtr2 fulfils an analogous, function to that of human p15 in maintaining the structural integrity of, the heterodimer. In addition, Mtr2 presents a long internal loop, which, contains residues that affect the export of the large ribosomal subunit.
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<StructureSection load='1of5' size='340' side='right'caption='[[1of5]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1of5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of5 OCA], [https://pdbe.org/1of5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of5 RCSB], [https://www.ebi.ac.uk/pdbsum/1of5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1of5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1of5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The association between Mtr2 and Mex67 is essential for the nuclear export of bulk messenger RNA in yeast. In metazoans, the analogous function is carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly conserved proteins, their binding partners, Mtr2 and p15, share no sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like family and interacts with Mex67, forming a complex with a similar structural architecture to that of TAP-p15. Mtr2 fulfils an analogous function to that of human p15 in maintaining the structural integrity of the heterodimer. In addition, Mtr2 presents a long internal loop, which contains residues that affect the export of the large ribosomal subunit.
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==About this Structure==
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Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15.,Fribourg S, Conti E EMBO Rep. 2003 Jul;4(7):699-703. PMID:12835756<ref>PMID:12835756</ref>
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1OF5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Hg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15., Fribourg S, Conti E, EMBO Rep. 2003 Jul;4(7):699-703. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12835756 12835756]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1of5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Conti, E.]]
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[[Category: Conti E]]
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[[Category: Fribourg, S.]]
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[[Category: Fribourg S]]
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[[Category: HG]]
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[[Category: leucine-rich repeat]]
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[[Category: mrna transport]]
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[[Category: nuclear protein]]
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[[Category: nuclear transport]]
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[[Category: repeat]]
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[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:57:18 2008''
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Current revision

Crystal structure of Mex67-Mtr2

PDB ID 1of5

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OCA

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