3rpm

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[[Image:3rpm.png|left|200px]]
 
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==Crystal structure of the first GH20 domain of a novel Beta-N-acetyl-hexosaminidase StrH from Streptococcus pneumoniae R6==
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The line below this paragraph, containing "STRUCTURE_3rpm", creates the "Structure Box" on the page.
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<StructureSection load='3rpm' size='340' side='right'caption='[[3rpm]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3rpm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RPM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3rpm| PDB=3rpm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rpm OCA], [https://pdbe.org/3rpm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rpm RCSB], [https://www.ebi.ac.uk/pdbsum/3rpm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rpm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8DRL6_STRR6 Q8DRL6_STRR6]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The beta-N-acetylhexosaminidase (EC 3.2.1.52) from glycoside hydrolase family 20 (GH20) catalyzes the hydrolysis of the beta-N-acetylglucosamine (NAG) group from the nonreducing end of various glycoconjugates. The putative surface-exposed N-acetylhexosaminidase StrH/Spr0057 from Streptococcus pneumoniae R6 was proved to contribute to the virulence by removal of beta(1,2)-linked NAG on host defense molecules following the cleavage of sialic acid and galactose by neuraminidase and beta-galactosidase, respectively. StrH is the only reported GH20 enzyme that contains a tandem repeat of two 53% sequence-identical catalytic domains (designated as GH20-1 and GH20-2, respectively). Here, we present the 2.1 A crystal structure of the N-terminal domain of StrH (residues Glu-175 to Lys-642) complexed with NAG. It adopts an overall structure similar to other GH20 enzymes: a (beta/alpha)(8) TIM barrel with the active site residing at the center of the beta-barrel convex side. The kinetic investigation using 4-nitrophenyl N-acetyl-beta-d-glucosaminide as the substrate demonstrated that GH20-1 had an enzymatic activity (k(cat)/K(m)) of one-fourth compared with GH20-2. The lower activity of GH20-1 could be attributed to the substitution of active site Cys-469 of GH20-1 to the counterpart Tyr-903 of GH20-2. A complex model of NAGbeta(1,2)Man at the active site of GH20-1 combined with activity assays of the corresponding site-directed mutants characterized two key residues Trp-443 and Tyr-482 at subsite +1 of GH20-1 (Trp-876 and Tyr-914 of GH20-2) that might determine the beta(1,2) substrate specificity. Taken together, these findings shed light on the mechanism of catalytic specificity toward the beta(1,2)-linked beta-N-acetylglucosides.
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===Crystal structure of the first GH20 domain of a novel Beta-N-acetyl-hexosaminidase StrH from Streptococcus pneumoniae R6===
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Structural basis for the substrate specificity of a novel beta-N-acetylhexosaminidase StrH protein from Streptococcus pneumoniae R6.,Jiang YL, Yu WL, Zhang JW, Frolet C, Di Guilmi AM, Zhou CZ, Vernet T, Chen Y J Biol Chem. 2011 Dec 16;286(50):43004-12. doi: 10.1074/jbc.M111.256578. Epub, 2011 Oct 19. PMID:22013074<ref>PMID:22013074</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3rpm" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3rpm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPM OCA].
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*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
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[[Category: Beta-N-acetylhexosaminidase]]
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*[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]]
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[[Category: Streptococcus pneumoniae]]
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*[[Beta-N-acetylhexosaminidase 3D structures|Beta-N-acetylhexosaminidase 3D structures]]
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[[Category: Jiang, Y L.]]
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== References ==
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[[Category: Yu, W L.]]
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<references/>
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[[Category: Zhang, J W.]]
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__TOC__
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[[Category: Beta-n-acetyl-hexosaminidase]]
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</StructureSection>
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[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Tim barrel]]
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[[Category: Streptococcus pneumoniae R6]]
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[[Category: Jiang YL]]
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[[Category: Yu WL]]
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[[Category: Zhang JW]]

Current revision

Crystal structure of the first GH20 domain of a novel Beta-N-acetyl-hexosaminidase StrH from Streptococcus pneumoniae R6

PDB ID 3rpm

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