4a2i

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[[Image:4a2i.jpg|left|200px]]
 
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==Cryo-electron Microscopy Structure of the 30S Subunit in Complex with the YjeQ Biogenesis Factor==
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The line below this paragraph, containing "STRUCTURE_4a2i", creates the "Structure Box" on the page.
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<SX load='4a2i' size='340' side='right' viewer='molstar' caption='[[4a2i]], [[Resolution|resolution]] 16.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4a2i]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A2I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2i OCA], [https://pdbe.org/4a2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a2i RCSB], [https://www.ebi.ac.uk/pdbsum/4a2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2i ProSAT]</span></td></tr>
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{{STRUCTURE_4a2i| PDB=4a2i | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS17_ECOLI RS17_ECOLI] One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. Also plays a role in translational accuracy; neamine-resistant ribosomes show reduced neamine-induced misreading in vitro.[HAMAP-Rule:MF_01345]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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YjeQ is a protein broadly conserved in bacteria containing an N-terminal oligonucleotide/oligosaccharide fold (OB-fold) domain, a central GTPase domain, and a C-terminal zinc-finger domain. YjeQ binds tightly and stoichiometrically to the 30S subunit, which stimulates its GTPase activity by 160-fold. Despite growing evidence for the involvement of the YjeQ protein in bacterial 30S subunit assembly, the specific function and mechanism of this protein remain unclear. Here, we report the costructure of YjeQ with the 30S subunit obtained by cryo-electron microscopy. The costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit. This binding location of YjeQ in the 30S subunit suggests a chaperone role in processing of the 3' end of the rRNA as well as in mediating the correct orientation of the main domains of the 30S subunit. In addition, the YjeQ binding site partially overlaps with the interaction site of initiation factors 2 and 3, and upon binding, YjeQ covers three inter-subunit bridges that are important for the association of the 30S and 50S subunits. Hence, our structure suggests that YjeQ may assist in ribosome maturation by preventing premature formation of the translation initiation complex and association with the 50S subunit. Together, these results support a role for YjeQ in the late stages of 30S maturation.
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===CRYO-ELECTRON MICROSCOPY STRUCTURE OF THE 30S SUBUNIT IN COMPLEX WITH THE YJEQ BIOGENESIS FACTOR===
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Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.,Jomaa A, Stewart G, Mears JA, Kireeva I, Brown ED, Ortega J RNA. 2011 Nov;17(11):2026-38. Epub 2011 Sep 29. PMID:21960487<ref>PMID:21960487</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4a2i" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21960487}}, adds the Publication Abstract to the page
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21960487 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21960487}}
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__TOC__
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</SX>
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==About this Structure==
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[[4a2i]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2I OCA].
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==Reference==
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<ref group="xtra">PMID:021960487</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
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[[Category: Large Structures]]
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[[Category: Brown, E D.]]
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[[Category: Brown ED]]
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[[Category: Jomaa, A.]]
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[[Category: Jomaa A]]
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[[Category: Kireeva, I.]]
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[[Category: Kireeva I]]
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[[Category: Mears, J A.]]
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[[Category: Mears JA]]
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[[Category: Ortega, J.]]
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[[Category: Ortega J]]
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[[Category: Stewart, G.]]
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[[Category: Stewart G]]
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[[Category: Ribosome assembly]]
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[[Category: Ribosome-hydrolase complex]]
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Current revision

Cryo-electron Microscopy Structure of the 30S Subunit in Complex with the YjeQ Biogenesis Factor

4a2i, resolution 16.50Å

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