1pfd

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[[Image:1pfd.jpg|left|200px]]<br /><applet load="1pfd" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pfd" />
 
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'''THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES'''<br />
 
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==Overview==
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==THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES==
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The [2Fe-2S]ferredoxin I (Fd I) from parsley leaves (Mr = 10,500; 96 amino, acids) in the Fe(III)-Fe(III) oxidized form has been studied by 1H-NMR, spectroscopy. Sequence-specific 1H-NMR assignments were obtained through, two-dimensional classical double-quantum-filtered-COSY, NOESY and TOCSY, spectra. NOEs between protons as close as 5.6 A from the paramagnetic, Fe(III) atoms were observed at 800 MHz. A total of 3066 NOEs (of which, 2533 are meaningful) and 18 distance constraints taken from X-ray, crystallography of the Fe2S2 active site were used to obtain the solution, structure. From inversion recovery NOESY experiments, 33 longitudinal, relaxation rate (Qpara) constraints were used for the structural, refinement. The final structure was obtained by a process of restrained, energy minimization. Root-mean-square (rmsd) deviation values obtained for, the family of 18 structures (with reference to the average structure) are, 0.52 +/- 0.10 A and 0.91 +/- 0.12 A for backbone and all heavy atoms, respectively. The structure consists of seven-strands of beta-sheets and, four short alpha-helices. The quality of the present solution structure is, among the best of those reported for [2Fe-2S]ferredoxins. The secondary, structure and overall folding are compared with those of Anabaena, variabilis Fd and the higher plant Equistum arvense (horse tail) protein, determined through X-ray crystallography. The groups believed to be, responsible for electron transfer have been analysed.
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<StructureSection load='1pfd' size='340' side='right'caption='[[1pfd]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pfd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfd OCA], [https://pdbe.org/1pfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfd RCSB], [https://www.ebi.ac.uk/pdbsum/1pfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7M1S1_PETCR Q7M1S1_PETCR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/1pfd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pfd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The [2Fe-2S]ferredoxin I (Fd I) from parsley leaves (Mr = 10,500; 96 amino acids) in the Fe(III)-Fe(III) oxidized form has been studied by 1H-NMR spectroscopy. Sequence-specific 1H-NMR assignments were obtained through two-dimensional classical double-quantum-filtered-COSY, NOESY and TOCSY spectra. NOEs between protons as close as 5.6 A from the paramagnetic Fe(III) atoms were observed at 800 MHz. A total of 3066 NOEs (of which 2533 are meaningful) and 18 distance constraints taken from X-ray crystallography of the Fe2S2 active site were used to obtain the solution structure. From inversion recovery NOESY experiments, 33 longitudinal relaxation rate (Qpara) constraints were used for the structural refinement. The final structure was obtained by a process of restrained energy minimization. Root-mean-square (rmsd) deviation values obtained for the family of 18 structures (with reference to the average structure) are 0.52 +/- 0.10 A and 0.91 +/- 0.12 A for backbone and all heavy atoms respectively. The structure consists of seven-strands of beta-sheets and four short alpha-helices. The quality of the present solution structure is among the best of those reported for [2Fe-2S]ferredoxins. The secondary structure and overall folding are compared with those of Anabaena variabilis Fd and the higher plant Equistum arvense (horse tail) protein determined through X-ray crystallography. The groups believed to be responsible for electron transfer have been analysed.
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==About this Structure==
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The solution structure of parsley [2Fe-2S]ferredoxin.,Im SC, Liu G, Luchinat C, Sykes AG, Bertini I Eur J Biochem. 1998 Dec 1;258(2):465-77. PMID:9874213<ref>PMID:9874213</ref>
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1PFD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum] with <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--nitrate_reductase Ferredoxin--nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.7.2 1.7.7.2] Known structural/functional Site: <scene name='pdbsite=FES:Fe+Bind+Site'>FES</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The solution structure of parsley [2Fe-2S]ferredoxin., Im SC, Liu G, Luchinat C, Sykes AG, Bertini I, Eur J Biochem. 1998 Dec 1;258(2):465-77. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9874213 9874213]
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</div>
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[[Category: Ferredoxin--nitrate reductase]]
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<div class="pdbe-citations 1pfd" style="background-color:#fffaf0;"></div>
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[[Category: Petroselinum crispum]]
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[[Category: Single protein]]
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[[Category: Bertini, I.]]
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[[Category: Im, S.C.]]
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[[Category: Liu, G.]]
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[[Category: Luchinat, C.]]
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[[Category: Sykes, A.G.]]
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[[Category: FES]]
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[[Category: [2fe-2s] ferredoxin]]
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[[Category: electron transport]]
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[[Category: nuclear relaxation]]
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[[Category: paramagnetism]]
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[[Category: solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:59:51 2008''
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==See Also==
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Petroselinum crispum]]
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[[Category: Bertini I]]
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[[Category: Im S-C]]
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[[Category: Liu G]]
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[[Category: Luchinat C]]
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[[Category: Sykes AG]]

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THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES

PDB ID 1pfd

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