2lfk

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[[Image:2lfk.jpg|left|200px]]
 
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==NMR solution structure of native TdPI-short==
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The line below this paragraph, containing "STRUCTURE_2lfk", creates the "Structure Box" on the page.
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<StructureSection load='2lfk' size='340' side='right'caption='[[2lfk]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2lfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhipicephalus_appendiculatus Rhipicephalus appendiculatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LFK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lfk OCA], [https://pdbe.org/2lfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lfk RCSB], [https://www.ebi.ac.uk/pdbsum/2lfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lfk ProSAT]</span></td></tr>
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{{STRUCTURE_2lfk| PDB=2lfk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q1EG59_RHIAP Q1EG59_RHIAP]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tick-derived protease inhibitor (TdPI) is a tight-binding Kunitz-related inhibitor of human tryptase beta with a unique structure and disulfide-bond pattern. Here we analyzed its oxidative folding and reductive unfolding by chromatographic and disulfide analyses of acid-trapped intermediates. TdPI folds through a stepwise generation of heterogeneous populations of one-disulfide, two-disulfide, and three-disulfide intermediates, with a major accumulation of the nonnative three-disulfide species IIIa. The rate-limiting step of the process is disulfide reshuffling within the three-disulfide population towards a productive intermediate that oxidizes directly into the native four-disulfide protein. TdPI unfolds through a major accumulation of the native three-disulfide species IIIb and the subsequent formation of two-disulfide and one-disulfide intermediates. NMR characterization of the acid-trapped and further isolated IIIa intermediate revealed a highly disordered conformation that is maintained by the presence of the disulfide bonds. Conversely, the NMR structure of IIIb showed a native-like conformation, with three native disulfide bonds and increased flexibility only around the two free cysteines, thus providing a molecular basis for its role as a productive intermediate. Comparison of TdPI with a shortened variant lacking the flexible prehead and posthead segments revealed that these regions do not contribute to the protein conformational stability or the inhibition of trypsin but are important for both the initial steps of the folding reaction and the inhibition of tryptase beta. Taken together, the results provide insights into the mechanism of oxidative folding of Kunitz inhibitors and pave the way for the design of TdPI variants with improved properties for biomedical applications.
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===NMR solution structure of native TdPI-short===
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Oxidative Folding and Structural Analyses of a Kunitz-Related Inhibitor and Its Disulfide Intermediates: Functional Implications.,Bronsoms S, Pantoja-Uceda D, Gabrijelcic-Geiger D, Sanglas L, Aviles FX, Santoro J, Sommerhoff CP, Arolas JL J Mol Biol. 2011 Oct 18. PMID:22033478<ref>PMID:22033478</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2lfk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22033478}}, adds the Publication Abstract to the page
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*[[Tryptase inhibitor|Tryptase inhibitor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22033478 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22033478}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2lfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhipicephalus_appendiculatus Rhipicephalus appendiculatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFK OCA].
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==Reference==
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<ref group="xtra">PMID:022033478</ref><references group="xtra"/>
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[[Category: Rhipicephalus appendiculatus]]
[[Category: Rhipicephalus appendiculatus]]
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[[Category: Arolas, J.]]
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[[Category: Arolas J]]
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[[Category: Aviles, F.]]
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[[Category: Aviles F]]
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[[Category: Bronsoms, S.]]
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[[Category: Bronsoms S]]
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[[Category: Gabrijelcic-Geiger, D.]]
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[[Category: Gabrijelcic-Geiger D]]
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[[Category: Pantoja-Uceda, D.]]
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[[Category: Pantoja-Uceda D]]
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[[Category: Sanglas, L.]]
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[[Category: Sanglas L]]
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[[Category: Santoro, J.]]
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[[Category: Santoro J]]
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[[Category: Sommerhoff, C.]]
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[[Category: Sommerhoff C]]
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[[Category: Hydrolase inhibitor]]
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Current revision

NMR solution structure of native TdPI-short

PDB ID 2lfk

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