3l1x

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[[Image:3l1x.png|left|200px]]
 
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==Crystal Structure of U-box Domain of Human E4B Ubiquitin Ligase==
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The line below this paragraph, containing "STRUCTURE_3l1x", creates the "Structure Box" on the page.
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<StructureSection load='3l1x' size='340' side='right'caption='[[3l1x]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3l1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L1X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l1x OCA], [https://pdbe.org/3l1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l1x RCSB], [https://www.ebi.ac.uk/pdbsum/3l1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l1x ProSAT]</span></td></tr>
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{{STRUCTURE_3l1x| PDB=3l1x | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBE4B_HUMAN UBE4B_HUMAN] Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/3l1x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l1x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human E4B, also called UFD2a, is a U box-containing protein that functions as an E3 ubiquitin ligase and an E4 polyubiquitin chain elongation factor. E4B is thought to participate in the proteasomal degradation of misfolded or damaged proteins through association with chaperones. The U box domain is an anchor site for E2 ubiquitin-conjugating enzymes, but little is known of the binding mechanism. Using X-ray crystallography and NMR spectroscopy, we determined the structures of E4B U box free and bound to UbcH5c and Ubc4 E2s. Whereas previously characterized U box domains are homodimeric, we show that E4B U box is a monomer stabilized by a network of hydrogen bonds identified from scalar coupling measurements. These structural studies, complemented by calorimetry- and NMR-based binding assays, suggest an allosteric regulation of UbcH5c and Ubc4 by E4B U box and provide a molecular basis to understand how the ubiquitylation machinery involving E4B assembles.
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===Crystal Structure of U-box Domain of Human E4B Ubiquitin Ligase===
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Molecular basis for the association of human E4B U box ubiquitin ligase with E2-conjugating enzymes UbcH5c and Ubc4.,Benirschke RC, Thompson JR, Nomine Y, Wasielewski E, Juranic N, Macura S, Hatakeyama S, Nakayama KI, Botuyan MV, Mer G Structure. 2010 Aug 11;18(8):955-65. PMID:20696396<ref>PMID:20696396</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20696396}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3l1x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20696396 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20696396}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3l1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L1X OCA].
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==Reference==
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<ref group="xtra">PMID:020696396</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Benirschke, R.]]
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[[Category: Large Structures]]
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[[Category: Mer, G.]]
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[[Category: Benirschke R]]
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[[Category: Thompson, J R.]]
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[[Category: Mer G]]
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[[Category: E3 ubiquitin ligase]]
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[[Category: Thompson JR]]
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[[Category: E4 ubiquitin ligase]]
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[[Category: Ligase]]
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[[Category: U-box domain]]
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[[Category: Ubl conjugation pathway]]
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Current revision

Crystal Structure of U-box Domain of Human E4B Ubiquitin Ligase

PDB ID 3l1x

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