1e60

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[[Image:1e60.gif|left|200px]]<br />
 
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<applet load="1e60" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e60, resolution 2.0&Aring;" />
 
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'''OXIDIZED DMSO REDUCTASE EXPOSED TO HEPES-STRUCTURE II BUFFER'''<br />
 
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==Overview==
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==OXIDIZED DMSO REDUCTASE EXPOSED TO HEPES - Structure II BUFFER==
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Much is unknown concerning the role of thiolate ligands of molybdenum in, molybdopterin enzymes. It has been suggested that thiolate dissociation, from molybdenum is part of the catalytic mechanism of bis-molybdopterin, enzymes of the dimethyl sulfoxide reductase (DMSOR) family. For DMSOR from, Rhodobacter capsulatus, thiolate dissociation has therefore been, investigated crystallographically, by UV/visible spectroscopy, and by, enzyme assays. When crystallized from sodium citrate, all four thiolates, of DMSOR are within bonding distance of Mo, but after extended exposure to, Na(+)-Hepes, a pair of thiolates dissociates, a mixture of structures, being indicated after shorter exposures to this buffer. DMSOR is stable in, sodium citrate and other buffers but unstable aerobically although ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10985771 (full description)]]
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<StructureSection load='1e60' size='340' side='right'caption='[[1e60]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1e60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E60 FirstGlance]. <br>
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1E60 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]] with SO4, PGD and 2MO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: MOA and MOC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E60 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MO:MOLYBDENUM+(IV)OXIDE'>2MO</scene>, <scene name='pdbligand=PGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>PGD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e60 OCA], [https://pdbe.org/1e60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e60 RCSB], [https://www.ebi.ac.uk/pdbsum/1e60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e60 ProSAT]</span></td></tr>
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Reversible dissociation of thiolate ligands from molybdenum in an enzyme of the dimethyl sulfoxide reductase family., Bray RC, Adams B, Smith AT, Bennett B, Bailey S, Biochemistry. 2000 Sep 19;39(37):11258-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10985771 10985771]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DSTOR_RHOCA DSTOR_RHOCA] Catalyzes the reduction of dimethyl sulfoxide (DMSO) and trimethylamine N-oxide (TMAO) to dimethyl sulfide (DMS) and trimethylamine, respectively. The terminal DMSO reductase can also use various sulfoxides and N-oxide compounds as terminal electron acceptor in addition to DMSO and TMAO.<ref>PMID:2001248</ref> <ref>PMID:8856102</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e6/1e60_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e60 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rhodobacter capsulatus]]
[[Category: Rhodobacter capsulatus]]
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[[Category: Single protein]]
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[[Category: Adams B]]
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[[Category: Adams, B.]]
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[[Category: Bailey S]]
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[[Category: Bailey, S.]]
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[[Category: Bennett B]]
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[[Category: Bennett, B.]]
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[[Category: Bray RC]]
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[[Category: Bray, R.C.]]
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[[Category: Smith AT]]
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[[Category: Smith, A.T.]]
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[[Category: 2MO]]
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[[Category: PGD]]
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[[Category: SO4]]
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[[Category: dmso]]
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[[Category: molybdopterin]]
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[[Category: oxidoreductase]]
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[[Category: reductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:39:30 2007''
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Current revision

OXIDIZED DMSO REDUCTASE EXPOSED TO HEPES - Structure II BUFFER

PDB ID 1e60

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