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1xpa

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[[Image:1xpa.jpg|left|200px]]<br /><applet load="1xpa" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xpa" />
 
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'''SOLUTION STRUCTURE OF THE DNA-AND RPA-BINDING DOMAIN OF THE HUMAN REPAIR FACTOR XPA, NMR, 1 STRUCTURE'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE DNA-AND RPA-BINDING DOMAIN OF THE HUMAN REPAIR FACTOR XPA, NMR, 1 STRUCTURE==
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The solution structure of the central domain of the human nucleotide, excision repair protein XPA, which binds to damaged DNA and replication, protein A (RPA), was determined by nuclear magnetic resonance (NMR), spectroscopy. The central domain consists of a zinc-containing subdomain, and a C-terminal subdomain. The zinc-containing subdomain has a compact, globular structure and is distinct from the zinc-fingers found in, transcription factors. The C-terminal subdomain folds into a novel, alpha/beta structure with a positively charged superficial cleft. From the, NMR spectra of the complexes, DNA and RPA binding surfaces are suggested.
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<StructureSection load='1xpa' size='340' side='right'caption='[[1xpa]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xpa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XPA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xpa OCA], [https://pdbe.org/1xpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xpa RCSB], [https://www.ebi.ac.uk/pdbsum/1xpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xpa ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/XPA_HUMAN XPA_HUMAN] Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:[https://omim.org/entry/278700 278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.<ref>PMID:1339397</ref> <ref>PMID:1372103</ref> <ref>PMID:9671271</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/XPA_HUMAN XPA_HUMAN] Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.<ref>PMID:19197159</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xp/1xpa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xpa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of the central domain of the human nucleotide excision repair protein XPA, which binds to damaged DNA and replication protein A (RPA), was determined by nuclear magnetic resonance (NMR) spectroscopy. The central domain consists of a zinc-containing subdomain and a C-terminal subdomain. The zinc-containing subdomain has a compact globular structure and is distinct from the zinc-fingers found in transcription factors. The C-terminal subdomain folds into a novel alpha/beta structure with a positively charged superficial cleft. From the NMR spectra of the complexes, DNA and RPA binding surfaces are suggested.
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==Disease==
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Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA.,Ikegami T, Kuraoka I, Saijo M, Kodo N, Kyogoku Y, Morikawa K, Tanaka K, Shirakawa M Nat Struct Biol. 1998 Aug;5(8):701-6. PMID:9699634<ref>PMID:9699634</ref>
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Known diseases associated with this structure: Xeroderma pigmentosum, group A OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611153 611153]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1XPA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=NUL:Zn+Binding+Site'>NUL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XPA OCA].
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</div>
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<div class="pdbe-citations 1xpa" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA., Ikegami T, Kuraoka I, Saijo M, Kodo N, Kyogoku Y, Morikawa K, Tanaka K, Shirakawa M, Nat Struct Biol. 1998 Aug;5(8):701-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9699634 9699634]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ikegami, T.]]
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[[Category: Ikegami T]]
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[[Category: Kodo, N.]]
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[[Category: Kodo N]]
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[[Category: Kuraoka, I.]]
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[[Category: Kuraoka I]]
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[[Category: Kyogoku, Y.]]
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[[Category: Kyogoku Y]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Saijo, M.]]
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[[Category: Saijo M]]
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[[Category: Shirakawa, M.]]
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[[Category: Shirakawa M]]
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[[Category: Tanaka, K.]]
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[[Category: Tanaka K]]
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[[Category: ZN]]
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[[Category: dna repair]]
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[[Category: nucleotide excision repair]]
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[[Category: zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:22:07 2008''
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Current revision

SOLUTION STRUCTURE OF THE DNA-AND RPA-BINDING DOMAIN OF THE HUMAN REPAIR FACTOR XPA, NMR, 1 STRUCTURE

PDB ID 1xpa

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