2bkw
From Proteopedia
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| - | [[Image:2bkw.gif|left|200px]]<br /><applet load="2bkw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2bkw, resolution 2.57Å" /> | ||
| - | '''YEAST ALANINE:GLYOXYLATE AMINOTRANSFERASE YFL030W'''<br /> | ||
| - | == | + | ==Yeast alanine:glyoxylate aminotransferase YFL030w== |
| - | + | <StructureSection load='2bkw' size='340' side='right'caption='[[2bkw]], [[Resolution|resolution]] 2.57Å' scene=''> | |
| - | [ | + | == Structural highlights == |
| - | [ | + | <table><tr><td colspan='2'>[[2bkw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKW FirstGlance]. <br> |
| - | [ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |
| - | [ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkw OCA], [https://pdbe.org/2bkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bkw RCSB], [https://www.ebi.ac.uk/pdbsum/2bkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bkw ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | [[ | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/AGX1_YEAST AGX1_YEAST] Has alanine:glyoxylate aminotransferase activity.<ref>PMID:3933486</ref> <ref>PMID:16226833</ref> | |
| - | + | == Evolutionary Conservation == | |
| - | [ | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | [[ | + | Check<jmol> |
| - | [ | + | <jmolCheckbox> |
| - | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bkw_consurf.spt"</scriptWhenChecked> | |
| - | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |
| - | + | <text>to colour the structure by Evolutionary Conservation</text> | |
| - | + | </jmolCheckbox> | |
| - | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bkw ConSurf]. | |
| - | + | <div style="clear:both"></div> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| + | == Publication Abstract from PubMed == | ||
| + | We have determined the three-dimensional crystal structure of the protein encoded by the open reading frame YFL030w from Saccharomyces cerevisiae to a resolution of 2.6 A using single wavelength anomalous diffraction. YFL030w is a 385 amino-acid protein with sequence similarity to the aminotransferase family. The structure of the protein reveals a homodimer adopting the fold-type I of pyridoxal 5'-phosphate (PLP)-dependent aminotransferases. The PLP co-factor is covalently bound to the active site in the crystal structure. The protein shows close structural resemblance with the human alanine:glyoxylate aminotransferase (EC 2.6.1.44), an enzyme involved in the hereditary kidney stone disease primary hyperoxaluria type 1. In this paper we show that YFL030w codes for an alanine:glyoxylate aminotransferase, highly specific for its amino donor and acceptor substrates. | ||
| - | + | Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein.,Meyer P, Liger D, Leulliot N, Quevillon-Cheruel S, Zhou CZ, Borel F, Ferrer JL, Poupon A, Janin J, van Tilbeurgh H Biochimie. 2005 Dec;87(12):1041-7. Epub 2005 Oct 5. PMID:16226833<ref>PMID:16226833</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2bkw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Borel F]] | ||
| + | [[Category: Ferrer JL]] | ||
| + | [[Category: Janin J]] | ||
| + | [[Category: Leulliot N]] | ||
| + | [[Category: Liger D]] | ||
| + | [[Category: Meyer P]] | ||
| + | [[Category: Poupon A]] | ||
| + | [[Category: Quevillon-Cheruel S]] | ||
| + | [[Category: Zhou CZ]] | ||
| + | [[Category: Van Tilbeurgh H]] | ||
Current revision
Yeast alanine:glyoxylate aminotransferase YFL030w
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