2bl1

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[[Image:2bl1.gif|left|200px]]<br /><applet load="2bl1" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bl1, resolution 2.&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A PUTATIVE PHOSPHINOTHRICIN ACETYLTRANSFERASE (PA4866) FROM PSEUDOMONAS AERUGINOSA PAC1'''<br />
 
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==Overview==
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==Crystal structure of a putative phosphinothricin Acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1==
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Modification of the lipid A moiety of lipopolysaccharide by the addition, of the sugar 4-amino-4-deoxy-L-arabinose (L-Ara4N) is a strategy adopted, by pathogenic Gram-negative bacteria to evade cationic antimicrobial, peptides produced by the innate immune system. L-Ara4N biosynthesis is, therefore a potential anti-infective target, because inhibiting its, synthesis would render certain pathogens more sensitive to the immune, system. The bifunctional enzyme ArnA, which is required for L-Ara4N, biosynthesis, catalyzes the NAD(+)-dependent oxidative decarboxylation of, UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also, catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to, the 4'-amine of UDP-L-Ara4N. We now report the crystal structure of the, N-terminal formyltransferase domain in a complex with uridine, monophosphate and N-5-formyltetrahydrofolate. Using this structure, we, identify the active site of formyltransfer in ArnA, including the key, catalytic residues Asn(102), His(104), and Asp(140). Additionally, we have, shown that residues Ser(433) and Glu(434) of the decarboxylase domain are, required for the oxidative decarboxylation of UDP-GlcUA. An E434Q mutant, is inactive, suggesting that chemical rather than steric properties of, this residue are crucial in the decarboxylation reaction. Our data suggest, that the decarboxylase domain catalyzes both hydride abstraction, (oxidation) from the C-4' position and the subsequent decarboxylation.
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<StructureSection load='2bl1' size='340' side='right'caption='[[2bl1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BL1 FirstGlance]. <br>
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2BL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=AZI:'>AZI</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL1 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bl1 OCA], [https://pdbe.org/2bl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bl1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bl1 ProSAT]</span></td></tr>
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Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis., Williams GJ, Breazeale SD, Raetz CR, Naismith JH, J Biol Chem. 2005 Jun 17;280(24):23000-8. Epub 2005 Apr 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15809294 15809294]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MDDA_PSEAE MDDA_PSEAE] Plays a role in the resistance against the toxic effects of L-methionine sulfoximine (MSX), a rare amino acid, which inhibits glutamine synthetase (GlnA). Catalyzes the acetylation of L-methionine sulfoximine (MSX).[UniProtKB:A6VCX3]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2bl1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bl1 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Agha R]]
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[[Category: Agha, R.]]
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[[Category: Brown PR]]
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[[Category: Brown, P.R.]]
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[[Category: Davies AM]]
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[[Category: Davies, A.M.]]
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[[Category: Sutton BJ]]
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[[Category: Sutton, B.J.]]
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[[Category: Tata R]]
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[[Category: TATA, R.]]
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[[Category: AZI]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: gcn5 family]]
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[[Category: gnat]]
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[[Category: hypothetical protein]]
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[[Category: n-acetyltransferase]]
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[[Category: phosphinothricin]]
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[[Category: pseudomonas aeruginosa]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:25:20 2008''
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Current revision

Crystal structure of a putative phosphinothricin Acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1

PDB ID 2bl1

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OCA

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