2btq

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[[Image:2btq.gif|left|200px]]<br /><applet load="2btq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2btq, resolution 3.2&Aring;" />
 
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'''STRUCTURE OF BTUBAB HETERODIMER FROM PROSTHECOBACTER DEJONGEII'''<br />
 
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==Overview==
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==Structure of BtubAB heterodimer from Prosthecobacter dejongeii==
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alphabeta-Tubulin heterodimers, from which the microtubules of the, cytoskeleton are built, have a complex chaperone-dependent folding, pathway. They are thought to be unique to eukaryotes, whereas the, homologue FtsZ can be found in bacteria. The exceptions are BtubA and, BtubB from Prosthecobacter, which have higher sequence homology to, eukaryotic tubulin than to FtsZ. Here we show that some of their, properties are different from tubulin, such as weak dimerization and, chaperone-independent folding. However, their structure is strikingly, similar to tubulin including surface loops, and BtubA/B form tubulin-like, protofilaments. Presumably, BtubA/B were transferred from a eukaryotic, cell by horizontal gene transfer because their high degree of similarity, to eukaryotic genes is unique within the Prosthecobacter genome.
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<StructureSection load='2btq' size='340' side='right'caption='[[2btq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2btq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Prosthecobacter_dejongeii Prosthecobacter dejongeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BTQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2btq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2btq OCA], [https://pdbe.org/2btq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2btq RCSB], [https://www.ebi.ac.uk/pdbsum/2btq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2btq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GCC5_9BACT Q8GCC5_9BACT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bt/2btq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2btq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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alphabeta-Tubulin heterodimers, from which the microtubules of the cytoskeleton are built, have a complex chaperone-dependent folding pathway. They are thought to be unique to eukaryotes, whereas the homologue FtsZ can be found in bacteria. The exceptions are BtubA and BtubB from Prosthecobacter, which have higher sequence homology to eukaryotic tubulin than to FtsZ. Here we show that some of their properties are different from tubulin, such as weak dimerization and chaperone-independent folding. However, their structure is strikingly similar to tubulin including surface loops, and BtubA/B form tubulin-like protofilaments. Presumably, BtubA/B were transferred from a eukaryotic cell by horizontal gene transfer because their high degree of similarity to eukaryotic genes is unique within the Prosthecobacter genome.
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==About this Structure==
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Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.,Schlieper D, Oliva MA, Andreu JM, Lowe J Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9170-5. Epub 2005 Jun 20. PMID:15967998<ref>PMID:15967998</ref>
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2BTQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Prosthecobacter_dejongeii Prosthecobacter dejongeii] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BTQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer., Schlieper D, Oliva MA, Andreu JM, Lowe J, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9170-5. Epub 2005 Jun 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15967998 15967998]
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</div>
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[[Category: Prosthecobacter dejongeii]]
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<div class="pdbe-citations 2btq" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Lowe, J.]]
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[[Category: Schlieper, D.]]
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[[Category: GDP]]
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[[Category: SO4]]
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[[Category: bacterial tubulin]]
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[[Category: cytoskeletal protein]]
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[[Category: cytoskeleton]]
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[[Category: polymerization]]
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[[Category: protein complex]]
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[[Category: verrucomicrobia]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:27:42 2008''
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==See Also==
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*[[BtuB 3D structures|BtuB 3D structures]]
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Prosthecobacter dejongeii]]
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[[Category: Lowe J]]
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[[Category: Schlieper D]]

Current revision

Structure of BtubAB heterodimer from Prosthecobacter dejongeii

PDB ID 2btq

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