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2lc7

From Proteopedia

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[[Image:2lc7.jpg|left|200px]]
 
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==Solution structure of the isolated Par-6 PDZ domain==
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The line below this paragraph, containing "STRUCTURE_2lc7", creates the "Structure Box" on the page.
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<StructureSection load='2lc7' size='340' side='right'caption='[[2lc7]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2lc7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LC7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lc7 OCA], [https://pdbe.org/2lc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lc7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lc7 ProSAT]</span></td></tr>
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{{STRUCTURE_2lc7| PDB=2lc7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here, we report a novel mechanism of PDZ (PSD-95/Dlg/ZO-1) domain regulation that distorts a conserved element of PDZ ligand recognition. The polarity regulator Par-6 assembles a conserved multiprotein complex and is directly modulated by the Rho GTPase Cdc42. Cdc42 binds the adjacent Cdc42/Rac interactive binding (CRIB) and PDZ domains of Par-6, increasing C-terminal ligand binding affinity by 10-fold. By solving structures of the isolated PDZ domain and a disulfide-stabilized CRIB-PDZ, we detected a conformational switch that controls affinity by altering the configuration of the conserved "GLGF" loop. As a result, lysine 165 is displaced from the PDZ core by an adjacent hydrophobic residue, disrupting coordination of the PDZ ligand-binding cleft. Stabilization of the CRIB:PDZ interface restores K165 to its canonical location in the binding pocket. We conclude that a unique "dipeptide switch" in the Par-6 PDZ transmits a signal for allosteric activation to the ligand-binding pocket.
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===Solution structure of the isolated Par-6 PDZ domain===
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A Conformational Switch in the CRIB-PDZ Module of Par-6.,Whitney DS, Peterson FC, Volkman BF Structure. 2011 Nov 9;19(11):1711-22. PMID:22078569<ref>PMID:22078569</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2lc7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22078569 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22078569}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2lc7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC7 OCA].
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==Reference==
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<ref group="xtra">PMID:022078569</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Peterson, F C.]]
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[[Category: Large Structures]]
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[[Category: Volkman, B F.]]
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[[Category: Peterson FC]]
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[[Category: Whitney, D S.]]
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[[Category: Volkman BF]]
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[[Category: Allostery]]
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[[Category: Whitney DS]]
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[[Category: Cell adhesion]]
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[[Category: Cell polarity]]
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[[Category: Crib]]
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Current revision

Solution structure of the isolated Par-6 PDZ domain

PDB ID 2lc7

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