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3u1t
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Haloalkane Dehalogenase, DmmA, of marine microbial origin== | |
| + | <StructureSection load='3u1t' size='340' side='right'caption='[[3u1t]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3u1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U1T FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u1t OCA], [https://pdbe.org/3u1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u1t RCSB], [https://www.ebi.ac.uk/pdbsum/3u1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u1t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DmmA is a haloalkane dehalogenase (HLD) identified and characterized from the metagenomic DNA of a marine microbial consortium. Dehalogenase activity was detected with 1,3-dibromopropane as substrate, with steady-state kinetic parameters typical of HLDs (K(m) =0.24+/-0.05 mM, k(cat) =2.4+/-0.1 s-1). The 2.2-A crystal structure of DmmA revealed a fold and active site similar to other HLDs, but with a substantially larger active site binding pocket, suggestive of an ability to act on bulky substrates. This enhanced cavity was shown to accept a range of linear and cyclic substrates, suggesting that DmmA will contribute to the expanding industrial applications of HLDs. | ||
| - | + | Structure and activity of DmmA, a marine haloalkane dehalogenase.,Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL Protein Sci. 2011 Nov 28. doi: 10.1002/pro.2009. PMID:22124946<ref>PMID:22124946</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3u1t" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Dehalogenase 3D structures|Dehalogenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Haloalkane dehalogenase]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Miscellaneous nucleic acid]] | ||
| + | [[Category: Gehret, J J]] | ||
| + | [[Category: Smith, J L]] | ||
| + | [[Category: Alpha/beta-hydrolase]] | ||
| + | [[Category: Hydrolase]] | ||
Current revision
Haloalkane Dehalogenase, DmmA, of marine microbial origin
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