2c62

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[[Image:2c62.gif|left|200px]]<br /><applet load="2c62" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2c62, resolution 1.74&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE HUMAN TRANSCRIPTION COFACTOR PC4 IN COMPLEX WITH SINGLE-STRANDED DNA'''<br />
 
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==Overview==
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==Crystal Structure of the Human Transcription Cofactor PC4 in Complex with Single-Stranded DNA==
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The 1.74-A crystal structure of the human transcription cofactor PC4 in, complex with a single-stranded 20-mer oligonucleotide reveals how, symmetry-related beta-surfaces of the protein homodimer interact with, juxtaposed 5-nucleotide DNA regions running in opposite directions. The, structure explains high-affinity binding of PC4 to the complementary, strands of unwinding duplex DNA, and it suggests the cofactor may have a, role in relaxing negative supercoils or exposing unpaired bases for, sequence-specific recognition by other biomolecules.
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<StructureSection load='2c62' size='340' side='right'caption='[[2c62]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2c62]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C62 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c62 OCA], [https://pdbe.org/2c62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c62 RCSB], [https://www.ebi.ac.uk/pdbsum/2c62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c62 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TCP4_HUMAN TCP4_HUMAN] General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).<ref>PMID:8062392</ref> <ref>PMID:8062391</ref> <ref>PMID:7628453</ref> <ref>PMID:9482861</ref> <ref>PMID:16689930</ref> <ref>PMID:9360603</ref> <ref>PMID:16605275</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c6/2c62_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c62 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 1.74-A crystal structure of the human transcription cofactor PC4 in complex with a single-stranded 20-mer oligonucleotide reveals how symmetry-related beta-surfaces of the protein homodimer interact with juxtaposed 5-nucleotide DNA regions running in opposite directions. The structure explains high-affinity binding of PC4 to the complementary strands of unwinding duplex DNA, and it suggests the cofactor may have a role in relaxing negative supercoils or exposing unpaired bases for sequence-specific recognition by other biomolecules.
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==About this Structure==
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A global transcription cofactor bound to juxtaposed strands of unwound DNA.,Werten S, Moras D Nat Struct Mol Biol. 2006 Feb;13(2):181-2. Epub 2006 Jan 15. PMID:16415882<ref>PMID:16415882</ref>
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2C62 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C62 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A global transcription cofactor bound to juxtaposed strands of unwound DNA., Werten S, Moras D, Nat Struct Mol Biol. 2006 Feb;13(2):181-2. Epub 2006 Jan 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16415882 16415882]
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</div>
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<div class="pdbe-citations 2c62" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Werten, S.]]
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[[Category: Werten S]]
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[[Category: SO4]]
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[[Category: activator]]
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[[Category: dna unwinding]]
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[[Category: dna-binding]]
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[[Category: nuclear protein]]
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[[Category: phosphorylation]]
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[[Category: protein-dna complex]]
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[[Category: single-stranded dna]]
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[[Category: transcription]]
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[[Category: transcription cofactor]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:31:54 2008''
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Current revision

Crystal Structure of the Human Transcription Cofactor PC4 in Complex with Single-Stranded DNA

PDB ID 2c62

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