3pf4

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[[Image:3pf4.png|left|200px]]
 
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==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==
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The line below this paragraph, containing "STRUCTURE_3pf4", creates the "Structure Box" on the page.
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<StructureSection load='3pf4' size='340' side='right'caption='[[3pf4]], [[Resolution|resolution]] 1.38&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3pf4| PDB=3pf4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [https://pdbe.org/3pf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial cold shock proteins (CSPs) regulate the cellular response to temperature downshift. Their general principle of function involves RNA chaperoning and transcriptional antitermination. Here we present two crystal structures of cold shock protein B from Bacillus subtilis (Bs-CspB) in complex with either a hexanucleotide (5'-UUUUUU-3') or heptanucleotide (5'-GUCUUUA-3') single-stranded RNA (ssRNA). Hydrogen bonds and stacking interactions between RNA bases and aromatic sidechains characterize individual binding subsites. Additional binding subsites which are not occupied by the ligand in the crystal structure were revealed by NMR spectroscopy in solution on Bs-CspB.RNA complexes. Binding studies demonstrate that Bs-CspB associates with ssDNA as well as ssRNA with moderate sequence specificity. Varying affinities of oligonucleotides are reflected mainly in changes of the dissociation rates. The generally lower binding affinity of ssRNA compared to its ssDNA analog is attributed solely to the substitution of thymine by uracil bases in RNA.
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===Crystal structure of Bs-CspB in complex with r(GUCUUUA)===
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RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.,Sachs R, Max KE, Heinemann U, Balbach J RNA. 2011 Nov 29. PMID:22128343<ref>PMID:22128343</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pf4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22128343 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22128343}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA].
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==Reference==
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<ref group="xtra">PMID:022128343</ref><references group="xtra"/>
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Heinemann, U.]]
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[[Category: Large Structures]]
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[[Category: Max, K E.A.]]
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[[Category: Heinemann U]]
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[[Category: Sachs, R.]]
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[[Category: Max KEA]]
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[[Category: Beta barrel]]
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[[Category: Sachs R]]
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[[Category: Cold shock domain]]
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[[Category: Cold shock response]]
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[[Category: Cytosol]]
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[[Category: Gene regulation-rna complex]]
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[[Category: Ob fold]]
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[[Category: Protein-rna complex]]
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[[Category: Rna/dna binding]]
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[[Category: Single-stranded rna and dna]]
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[[Category: Transcription regulation]]
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[[Category: Translation regulation]]
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Current revision

Crystal structure of Bs-CspB in complex with r(GUCUUUA)

PDB ID 3pf4

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