3uai

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[[Image:3uai.jpg|left|200px]]
 
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==Structure of the Shq1-Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae==
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The line below this paragraph, containing "STRUCTURE_3uai", creates the "Structure Box" on the page.
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<StructureSection load='3uai' size='340' side='right'caption='[[3uai]], [[Resolution|resolution]] 3.06&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3uai]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.06&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uai OCA], [https://pdbe.org/3uai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uai RCSB], [https://www.ebi.ac.uk/pdbsum/3uai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uai ProSAT]</span></td></tr>
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{{STRUCTURE_3uai| PDB=3uai | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CBF5_YEAST CBF5_YEAST] Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. May function as a pseudouridine synthase. Binds in vitro to centromeres and microtubules. It is a centromeric DNA-CBF3-binding factor which is involved in mitotic chromosome segregation. Essential for cell growth.<ref>PMID:8336724</ref> <ref>PMID:9315678</ref> <ref>PMID:9472021</ref> <ref>PMID:9848653</ref> <ref>PMID:10523634</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Shq1 is a conserved protein required for the biogenesis of eukaryotic H/ACA ribonucleoproteins (RNPs), including human telomerase. We report the structure of the Shq1-specific domain alone and in complex with H/ACA RNP proteins Cbf5, Nop10 and Gar1. The Shq1-specific domain adopts a novel helical fold and primarily contacts the PUA domain and the otherwise disordered C-terminal extension (CTE) of Cbf5. The structure shows that dyskeratosis congenita mutations found in the CTE of human Cbf5 likely interfere with Shq1 binding. However, most mutations in the PUA domain are not located at the Shq1-binding surface and also have little effect on the yeast Cbf5-Shq1 interaction. Shq1 binds Cbf5 independently of the H/ACA RNP proteins Nop10, Gar1 and Nhp2 and the assembly factor Naf1, but shares an overlapping binding surface with H/ACA RNA. Shq1 point mutations that disrupt Cbf5 interaction suppress yeast growth particularly at elevated temperatures. Our results suggest that Shq1 functions as an assembly chaperone that protects the Cbf5 protein complexes from non-specific RNA binding and aggregation before assembly of H/ACA RNA.
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===Structure of the Shq1-Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae===
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Structure of the Shq1-Cbf5-Nop10-Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita.,Li S, Duan J, Li D, Ma S, Ye K EMBO J. 2011 Nov 25. doi: 10.1038/emboj.2011.427. PMID:22117216<ref>PMID:22117216</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3uai" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22117216 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22117216}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3uai]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAI OCA].
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Ye K]]
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==Reference==
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<ref group="xtra">PMID:022117216</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Ye, K.]]
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[[Category: H/aca rna]]
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[[Category: H/aca rnp assembly intermediate]]
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[[Category: Isomerase-chaperone complex]]
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[[Category: Nuclear]]
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Current revision

Structure of the Shq1-Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae

PDB ID 3uai

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