4a0k

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[[Image:4a0k.jpg|left|200px]]
 
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==STRUCTURE OF DDB1-DDB2-CUL4A-RBX1 BOUND TO A 12 BP ABASIC SITE CONTAINING DNA-DUPLEX==
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The line below this paragraph, containing "STRUCTURE_4a0k", creates the "Structure Box" on the page.
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<StructureSection load='4a0k' size='340' side='right'caption='[[4a0k]], [[Resolution|resolution]] 5.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4a0k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A0K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.93&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene></td></tr>
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{{STRUCTURE_4a0k| PDB=4a0k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a0k OCA], [https://pdbe.org/4a0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a0k RCSB], [https://www.ebi.ac.uk/pdbsum/4a0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a0k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CUL4A_HUMAN CUL4A_HUMAN] Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.<ref>PMID:14578910</ref> <ref>PMID:14609952</ref> <ref>PMID:15548678</ref> <ref>PMID:15448697</ref> <ref>PMID:16678110</ref> <ref>PMID:16537899</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DDB1-CUL4-RBX1 (CRL4) ubiquitin ligase family regulates a diverse set of cellular pathways through dedicated substrate receptors (DCAFs). The DCAF DDB2 detects UV-induced pyrimidine dimers in the genome and facilitates nucleotide excision repair. We provide the molecular basis for DDB2 receptor-mediated cyclobutane pyrimidine dimer recognition in chromatin. The structures of the fully assembled DDB1-DDB2-CUL4A/B-RBX1 (CRL4(DDB2)) ligases reveal that the mobility of the ligase arm creates a defined ubiquitination zone around the damage, which precludes direct ligase activation by DNA lesions. Instead, the COP9 signalosome (CSN) mediates the CRL4(DDB2) inhibition in a CSN5 independent, nonenzymatic, fashion. In turn, CSN inhibition is relieved upon DNA damage binding to the DDB2 module within CSN-CRL4(DDB2). The Cockayne syndrome A DCAF complex crystal structure shows that CRL4(DCAF(WD40)) ligases share common architectural features. Our data support a general mechanism of ligase activation, which is induced by CSN displacement from CRL4(DCAF) on substrate binding to the DCAF.
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===STRUCTURE OF DDB1-DDB2-CUL4A-RBX1 BOUND TO A 12 BP ABASIC SITE CONTAINING DNA-DUPLEX===
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The Molecular Basis of CRL4(DDB2/CSA) Ubiquitin Ligase Architecture, Targeting, and Activation.,Fischer ES, Scrima A, Bohm K, Matsumoto S, Lingaraju GM, Faty M, Yasuda T, Cavadini S, Wakasugi M, Hanaoka F, Iwai S, Gut H, Sugasawa K, Thoma NH Cell. 2011 Nov 23;147(5):1024-39. PMID:22118460<ref>PMID:22118460</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4a0k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22118460}}, adds the Publication Abstract to the page
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*[[Cullin 3D structures|Cullin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22118460 is the PubMed ID number.
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*[[DNA damage-binding protein|DNA damage-binding protein]]
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*[[Ring box protein 3D structures|Ring box protein 3D structures]]
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{{ABSTRACT_PUBMED_22118460}}
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*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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[[4a0k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A0K OCA].
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__TOC__
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022118460</ref><references group="xtra"/>
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[[Category: Danio rerio]]
[[Category: Danio rerio]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Fischer, E S.]]
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[[Category: Synthetic construct]]
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[[Category: Gut, H.]]
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[[Category: Fischer ES]]
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[[Category: Scrima, A.]]
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[[Category: Gut H]]
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[[Category: Thoma, N H.]]
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[[Category: Scrima A]]
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[[Category: Dna-binding protein-dna complex]]
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[[Category: Thoma NH]]
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[[Category: Ligase-dna-binding protein-dna complex]]
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Current revision

STRUCTURE OF DDB1-DDB2-CUL4A-RBX1 BOUND TO A 12 BP ABASIC SITE CONTAINING DNA-DUPLEX

PDB ID 4a0k

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