3u28

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[[Image:3u28.jpg|left|200px]]
 
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==Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae==
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The line below this paragraph, containing "STRUCTURE_3u28", creates the "Structure Box" on the page.
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<StructureSection load='3u28' size='340' side='right'caption='[[3u28]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3u28]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U28 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u28 OCA], [https://pdbe.org/3u28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u28 RCSB], [https://www.ebi.ac.uk/pdbsum/3u28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u28 ProSAT]</span></td></tr>
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{{STRUCTURE_3u28| PDB=3u28 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CBF5_YEAST CBF5_YEAST] Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. May function as a pseudouridine synthase. Binds in vitro to centromeres and microtubules. It is a centromeric DNA-CBF3-binding factor which is involved in mitotic chromosome segregation. Essential for cell growth.<ref>PMID:8336724</ref> <ref>PMID:9315678</ref> <ref>PMID:9472021</ref> <ref>PMID:9848653</ref> <ref>PMID:10523634</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Box H/ACA ribonucleoprotein particles (RNPs) mediate pseudouridine synthesis, ribosome formation, and telomere maintenance. The structure of eukaryotic H/ACA RNPs remains poorly understood. We reconstituted functional Saccharomyces cerevisiae H/ACA RNPs with recombinant proteins Cbf5, Nop10, Gar1, and Nhp2 and a two-hairpin H/ACA RNA; determined the crystal structure of a Cbf5, Nop10, and Gar1 ternary complex at 1.9 A resolution; and analyzed the structure-function relationship of the yeast complex. Although eukaryotic H/ACA RNAs have a conserved two-hairpin structure, isolated single-hairpin RNAs are also active in guiding pseudouridylation. Nhp2, unlike its archaeal counterpart, is largely dispensable for the activity, reflecting a functional adaptation of eukaryotic H/ACA RNPs to the variable RNA structure that Nhp2 binds. The N-terminal extension of Cbf5, a hot spot for dyskeratosis congenita mutation, forms an extra structural layer on the PUA domain. Gar1 is distinguished from the assembly factor Naf1 by containing a C-terminal extension that controls substrate turnover and the Gar1-Naf1 exchange during H/ACA RNP maturation. Our results reveal significant novel features of eukaryotic H/ACA RNPs.
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===Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae===
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Reconstitution and structural analysis of the yeast box H/ACA RNA-guided pseudouridine synthase.,Li S, Duan J, Li D, Yang B, Dong M, Ye K Genes Dev. 2011 Nov 15;25(22):2409-21. PMID:22085967<ref>PMID:22085967</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3u28" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22085967 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22085967}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3u28]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U28 OCA].
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Li S]]
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==Reference==
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[[Category: Ye K]]
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<ref group="xtra">PMID:022085967</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Li, S.]]
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[[Category: Ye, K.]]
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[[Category: H/aca rna]]
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[[Category: Isomerase-protein binding complex]]
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[[Category: Nucleolus]]
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[[Category: Pseudouridine synthase]]
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[[Category: Pseudouridylation]]
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Current revision

Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae

PDB ID 3u28

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