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Subtilisin

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[[Image:2z2x.png|left|200px|thumb|Crystal Structure of Subtilisin [[2z2x]]]]
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<StructureSection load='' size='350' side='right' caption='Subtilisin (deepskyblue) complex with streptomycin inhibitor (green) and Ca+2 ions (green) (PDB entry [[2sic]])' scene='43/430882/Cv/2'>
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{{STRUCTURE_2z2x| PDB=2z2x | SIZE=300| SCENE=Subtilisin/Cv/1 |right|CAPTION=Subtilisin with monoisopropylphosphorylserine complex with Ca+2 ions [[2z2x]] }}
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== Function ==
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[[Subtilisin]] is a serine protease. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzym<ref>PMID:4967581</ref>. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]].
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[[Subtilisin]] is a serine protease. It is widely used commercially in detergents.
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*'''Selenosubtilisin''' is a semisynthetic selenoenzyme produced by chemical modification of subtilisin<ref>PMID:8385489</ref>.<br />
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A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. The images at the left and at the right correspond to one representative Subtilisin, ''i.e.'' the crystal structure of native mature subtilisin from ''Thermococcus kodakarensis'' ([[2z2x]]). See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]].
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*'''Savinase''' is a subtilisin produced from a lentil<ref>PMID:24738747</ref>.<br />
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{{TOC limit|limit=2}}
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== Relevance ==
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Subtilisin is widely used commercially in detergents.
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== Structural highlights ==
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The active site of Sub contains the <scene name='43/430882/Cv/12'>catalytic triad: Ser-His-Asp</scene>. The <scene name='43/430882/Cv/13'>peptide inhibitor has numerous interactions with Sub</scene> and the <scene name='43/430882/Cv/14'>scissile bond is flanked by Cys-Pro-Met-Val</scene><ref>PMID:1920411</ref>.
== 3D Structures of Subtilisin ==
== 3D Structures of Subtilisin ==
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[[Subtilisin 3D structures]]
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''Updated December 2011''
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</StructureSection>
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=== Native mature subtilisin ===
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[[2z2x]] – TkSub - ''Thermococcus kodakarensis''<br />
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[[1sca]], [[1scb]], [[1sbc]] – BsSub - ''Bacillus subtilis'' (Subtilisin Carlsberg)<br />
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[[1pxq]] – BsSub - NMR<br />
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[[1gns]], [[1sup]], [[1st2]], [[1s01]], [[1sbt]], [[2sbt]], [[2st1]] – BaSub - ''Bacillus amyloliquefaciens''<br />
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[[1iav]], [[1gci]], [[1jea]], [[1st3]] – BlSub - ''Bacillus lentus'' (Subtilisin Savinase)<br />
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[[1ea7]], [[3d43]], [[2ixt]] – spehricase – Sub from ''Bacillus sphaericus''<br />
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[[2gko]] – Sub – psychrophilic ''Bacillus subtilis''
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=== Mutant mature subtilisin ===
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[[3f49]], [[3co0]], [[2zrq]] – TkSubb (mutant) <br />
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[[1gnv]], [[1dui]], [[1sue]], [[1a2q]], [[1aqn]], [[1sua]], [[1au9]], [[1ak9]], [[1yja]], [[1yjb]], [[1yjc]], [[1sbh]], [[1sbi]], [[1sub]], [[1suc]], [[1sud]], [[1s02]] – BaSub (mutant)<br />
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[[1ndu]], [[1q5p]], [[1c9j]], [[1c9m]], [[1c9n]] – BlSub (mutant)<br />
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[[2xrm]] - BcSub residues 19-321 - ''Bacillus clausii''<br />
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=== Unautoprocessed subtilisin (pro-Sub) ===
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[[2z2z]], [[2e1p]] – pro-TkSub <br />
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[[2zwo]], [[2zwp]] – pro-TKSub (mutant)<br />
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[[2wv7]] – pro-BcSub <br />
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[[2wwt]], [[2x8j]] - pro-BcSub (mutant)
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=== Mutant unautoprocessed subtilisin ===
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[[2zwo]], [[2zwp]] – pro-TKSub
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=== Mature subtilisin complexed with its propeptide ===
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[[3cnq]] – BaSub<br />
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[[1spb]] – BaSub (mutant)<br />
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[[3a3n]], [[3a3o]], [[3a3p]], [[2z56]], [[2z57]], [[2z58]], [[2z2y]], [[2z30]] – TkSub<br />
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[[1scj]] – BsSub
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=== Subtilisin complexed with polypeptide inhibitor ===
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[[1y1k]], [[1y33]], [[1y34]], [[1y3b]], [[1y3c]], [[1y3d]], [[1y3f]], [[1y48]], [[1y4a]], [[1y4d]], [[1tm3]], [[1tm4]],
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[[1tm1]], [[1lw6]] – BaSub+ chymotripsin inhibitor 2<br />
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[[1tm5]], [[1tm7]], [[1tmg]], [[1to1]], [[1to2]] - BaSub+ chymotrypsin inhibitor 2 (mutant)<br />
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[[3sic]], [[5sic]], [[2sic]] – BaSub+Streptomyces inhibitor SSI<br />
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[[1yu6]] – BsSub+ovomucoid <br />
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[[1v5i]] – BaSubb+POIA1 peptide <br />
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[[1tk2]], [[1ndq]] – BlSub+gramicidin S <br />
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[[1r0r]] – BsSub+ovomucoid<br />
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[[1oyv]] – BsSub+tomato inhibitor II<br />
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[[1sbn]], [[1sib]], [[2sec]], [[1sni]], [[1sce]] – BsSub+Elgin C<br />
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[[2sni]] - BaSub+Elgin C2 <br />
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[[1mee]] - BpSub+Elgin C – ''Bacillus pumilus''
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=== Subtilisin complexed with inhibitor ===
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[[3bgo]] – BaSub+azide <br />
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[[1c3l]] – BsSub+Xe<br />
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[[1be8]] – BsSub+cinnamoyl<br />
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[[1be6]] - BsSub+cinnamoyl+acetonitrile<br />
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[[1bfk]], [[1scd]] – BsSub+acetonitrile<br />
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[[1bfu]], [[1af4]] – BsSub+dioxane<br />
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[[1av7]], [[1avt]], [[3vsb]], [[1vsb]] – BsSub+boronic acid derivatives <br />
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[[1scn]] – BsSub+carbamate derivative <br />
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[[1bh6]] – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – ''Bacillus licheniformis''<br />
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[[2wuv]], [[2wuw]] – BlSub fragment+acetonitrile
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===Selenosubtilisin===
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[[1ubn]] – BaSel-Sub<br />
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[[1sel]] – BsSel-Sub
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===Savinase===
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[[3bx1]] – Sav – ''Bacillus lentus''
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Subtilisin (deepskyblue) complex with streptomycin inhibitor (green) and Ca+2 ions (green) (PDB entry 2sic)

Drag the structure with the mouse to rotate

References

  1. ↑ Smith EL, DeLange RJ, Evans WH, Landon M, Markland FS. Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships. J Biol Chem. 1968 May 10;243(9):2184-91. PMID:4967581
  2. ↑ Bell IM, Fisher ML, Wu ZP, Hilvert D. Kinetic studies on the peroxidase activity of selenosubtilisin. Biochemistry. 1993 Apr 13;32(14):3754-62. PMID:8385489
  3. ↑ Garcia-Mora P, Penas E, Frias J, Martinez-Villaluenga C. Savinase, the most suitable enzyme for releasing peptides from lentil (Lens culinaris var. Castellana) protein concentrates with multifunctional properties. J Agric Food Chem. 2014 May 7;62(18):4166-74. doi: 10.1021/jf500849u. Epub 2014, Apr 28. PMID:24738747 doi:http://dx.doi.org/10.1021/jf500849u
  4. ↑ Takeuchi Y, Satow Y, Nakamura KT, Mitsui Y. Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution. J Mol Biol. 1991 Sep 5;221(1):309-25. PMID:1920411

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