3nh2

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[[Image:3nh2.png|left|200px]]
 
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==Crystal structure of RNase T in complex with a stem DNA with a 3' overhang==
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<StructureSection load='3nh2' size='340' side='right'caption='[[3nh2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nh2]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NH2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nh2 OCA], [https://pdbe.org/3nh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nh2 RCSB], [https://www.ebi.ac.uk/pdbsum/3nh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nh2 ProSAT]</span></td></tr>
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{{STRUCTURE_3nh2| PDB=3nh2 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNT_ECOLI RNT_ECOLI] Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis, especially in strains lacking other exoribonucleases.[HAMAP-Rule:MF_00157]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases.
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===Crystal structure of RNase T in complex with a stem DNA with a 3' overhang===
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Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.,Hsiao YY, Yang CC, Lin CL, Lin JL, Duh Y, Yuan HS Nat Chem Biol. 2011 Feb 13. PMID:21317904<ref>PMID:21317904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3nh2" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21317904 is the PubMed ID number.
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{{ABSTRACT_PUBMED_21317904}}
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==About this Structure==
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[[3nh2]] is a 8 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NH2 OCA].
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==See Also==
==See Also==
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*[[Ribonuclease]]
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021317904</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Hsiao, Y Y.]]
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[[Category: Large Structures]]
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[[Category: Yuan, H S.]]
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[[Category: Synthetic construct]]
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[[Category: Exo-nuclease]]
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[[Category: Hsiao Y-Y]]
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[[Category: Exoribonuclease]]
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[[Category: Yuan HS]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: Protein-dna interaction]]
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[[Category: Rna maturation]]
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[[Category: Rna processing]]
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Current revision

Crystal structure of RNase T in complex with a stem DNA with a 3' overhang

PDB ID 3nh2

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