2j0a

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[[Image:2j0a.jpg|left|200px]]<br /><applet load="2j0a" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j0a, resolution 1.80&Aring;" />
 
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'''STRUCTURE OF THE CATALYTIC DOMAIN OF MOUSE MANIC FRINGE'''<br />
 
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==Overview==
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==Structure of the catalytic domain of mouse Manic Fringe==
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Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify, Notch receptors, altering their ligand-binding specificity to regulate, Notch signaling in development. We present the crystal structure of mouse, Manic Fringe bound to UDP and manganese. The structure reveals amino acid, residues involved in recognition of donor substrates and catalysis, and a, putative binding pocket for acceptor substrates. Mutations of several, invariant residues in this pocket impair Fringe activity in vivo.
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<StructureSection load='2j0a' size='340' side='right'caption='[[2j0a]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J0A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j0a OCA], [https://pdbe.org/2j0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j0a RCSB], [https://www.ebi.ac.uk/pdbsum/2j0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j0a ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j0a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify Notch receptors, altering their ligand-binding specificity to regulate Notch signaling in development. We present the crystal structure of mouse Manic Fringe bound to UDP and manganese. The structure reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several invariant residues in this pocket impair Fringe activity in vivo.
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==About this Structure==
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Structural insights into the Notch-modifying glycosyltransferase Fringe.,Jinek M, Chen YW, Clausen H, Cohen SM, Conti E Nat Struct Mol Biol. 2006 Oct;13(10):945-6. Epub 2006 Sep 10. PMID:16964258<ref>PMID:16964258</ref>
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2J0A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/O-fucosylpeptide_3-beta-N-acetylglucosaminyltransferase O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.222 2.4.1.222] Known structural/functional Site: <scene name='pdbsite=AC1:K+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0A OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural insights into the Notch-modifying glycosyltransferase Fringe., Jinek M, Chen YW, Clausen H, Cohen SM, Conti E, Nat Struct Mol Biol. 2006 Oct;13(10):945-6. Epub 2006 Sep 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16964258 16964258]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2j0a" style="background-color:#fffaf0;"></div>
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[[Category: O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase]]
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[[Category: Single protein]]
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[[Category: Chen, Y.W.]]
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[[Category: Clausen, H.]]
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[[Category: Cohen, S.M.]]
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[[Category: Conti, E.]]
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[[Category: Jinek, M.]]
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[[Category: K]]
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[[Category: SO4]]
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[[Category: developmental protein]]
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[[Category: glycoprotein]]
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[[Category: glycosyltransferase]]
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[[Category: golgi apparatus]]
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[[Category: membrane]]
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[[Category: notch signaling]]
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[[Category: signal-anchor]]
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[[Category: transferase]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:40:06 2008''
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==See Also==
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*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Chen Y-W]]
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[[Category: Clausen H]]
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[[Category: Cohen SM]]
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[[Category: Conti E]]
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[[Category: Jinek M]]

Current revision

Structure of the catalytic domain of mouse Manic Fringe

PDB ID 2j0a

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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