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3v6t
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3v6t is ON HOLD Authors: Deng, D., Yan, C.Y., Pan, X.J., Wang, J.W., Yan, N., Shi, Y.G. Description: Crystal structure of DNA-protein complex) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom== | |
| + | <StructureSection load='3v6t' size='340' side='right'caption='[[3v6t]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3v6t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas Xanthomonas]. The December 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TAL Effectors'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_12 10.2210/rcsb_pdb/mom_2014_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V6T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v6t OCA], [https://pdbe.org/3v6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v6t RCSB], [https://www.ebi.ac.uk/pdbsum/3v6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v6t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | TAL effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair using two hypervariable residues (known as RVD) at positions 12 and 13. Here, we report the crystal structures of a 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, super-helical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications. | ||
| - | + | Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.,Deng D, Yan C, Pan X, Mahfouz M, Wang J, Zhu JK, Shi Y, Yan N Science. 2012 Jan 5. PMID:22223738<ref>PMID:22223738</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3v6t" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[TAL effector|TAL effector]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: RCSB PDB Molecule of the Month]] | ||
| + | [[Category: TAL Effectors]] | ||
| + | [[Category: Xanthomonas]] | ||
| + | [[Category: Deng D]] | ||
| + | [[Category: Pan XJ]] | ||
| + | [[Category: Shi YG]] | ||
| + | [[Category: Wang JW]] | ||
| + | [[Category: Yan CY]] | ||
| + | [[Category: Yan N]] | ||
Current revision
Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom
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Categories: Large Structures | RCSB PDB Molecule of the Month | TAL Effectors | Xanthomonas | Deng D | Pan XJ | Shi YG | Wang JW | Yan CY | Yan N
