2j55

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[[Image:2j55.gif|left|200px]]<br /><applet load="2j55" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j55, resolution 2.15&Aring;" />
 
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'''X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE O-QUINONE IN COMPLEX WITH AMICYANIN.'''<br />
 
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==Overview==
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==X-ray reduced Paraccocus denitrificans methylamine dehydrogenase O- quinone in complex with amicyanin.==
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X-ray exposure during crystallographic data collection can result in, unintended redox changes in proteins containing functionally important, redox centers. In order to directly monitor X-ray-derived redox changes in, trapped oxidative half-reaction intermediates of Paracoccus denitrificans, methylamine dehydrogenase, a commercially available single-crystal UV/Vis, microspectrophotometer was installed on-line at the BioCARS beamline, 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox, state of the intermediates during X-ray exposure permitted the creation of, a general multi-crystal data collection strategy to generate true, structures of each redox intermediate.
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<StructureSection load='2j55' size='340' side='right'caption='[[2j55]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j55]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J55 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j55 OCA], [https://pdbe.org/2j55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j55 RCSB], [https://www.ebi.ac.uk/pdbsum/2j55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j55 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMCY_PARDE AMCY_PARDE] Primary acceptor of electrons from methylamine dehydrogenase. Passes those electrons on either a soluble cytochrome c or to pseudoazurin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j55_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j55 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2J55 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+L'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J55 OCA].
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*[[Amicyanin 3D structures|Amicyanin 3D structures]]
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*[[Methylamine dehydrogenase|Methylamine dehydrogenase]]
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==Reference==
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__TOC__
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Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17211075 17211075]
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</StructureSection>
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[[Category: Amine dehydrogenase]]
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[[Category: Large Structures]]
[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
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[[Category: Protein complex]]
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[[Category: Davidson VL]]
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[[Category: Davidson, V.]]
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[[Category: Pahl R]]
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[[Category: Pahl, R.]]
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[[Category: Pearson AR]]
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[[Category: Pearson, A.R.]]
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[[Category: Wilmot CM]]
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[[Category: Wilmot, C.M.]]
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[[Category: CU]]
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[[Category: GOL]]
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[[Category: copper]]
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[[Category: electron transport]]
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[[Category: metal-binding]]
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[[Category: methylamine dehydrogenase single crystal microspectrophoto]]
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[[Category: oxidoreductase]]
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[[Category: periplasmic]]
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[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:41:40 2008''
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Current revision

X-ray reduced Paraccocus denitrificans methylamine dehydrogenase O- quinone in complex with amicyanin.

PDB ID 2j55

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