3vhm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:48, 30 October 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3vhm.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of NPC-biotin-avidin complex==
-
The line below this paragraph, containing "STRUCTURE_3vhm", creates the "Structure Box" on the page.
+
<StructureSection load='3vhm' size='340' side='right'caption='[[3vhm]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3vhm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VHM FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NPK:5-[(3AS,4R,6AR)-1-{[(1R)-1-(6-NITRO-1,3-BENZODIOXOL-5-YL)ETHOXY]CARBONYL}-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOIC+ACID'>NPK</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_3vhm| PDB=3vhm | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vhm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vhm OCA], [https://pdbe.org/3vhm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vhm RCSB], [https://www.ebi.ac.uk/pdbsum/3vhm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vhm ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no report describing spatiotemporally controlled formation of the complex in live cells. Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity for (strept)avidin, but recover native affinity after UV irradiation. For application at the cell surface, we introduced an amine-reactive moiety into these "caged" biotin molecules. Specific fluorescence imaging of live cells that had been labeled with these agents and then UV-irradiated, was accomplished by addition of streptavidin conjugated with a fluorophore. We also demonstrated the applicability of these compounds for UV-irradiated-cell-specific drug delivery by using caged-biotin-labeled cells, a prodrug, and streptavidin conjugated with a prodrug-activating enzyme.
-
===Crystal structure of NPC-biotin-avidin complex===
+
Rational development of caged-biotin protein-labeling agents and some applications in live cells.,Terai T, Maki E, Sugiyama S, Takahashi Y, Matsumura H, Mori Y, Nagano T Chem Biol. 2011 Oct 28;18(10):1261-72. PMID:22035795<ref>PMID:22035795</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3vhm" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_22035795}}, adds the Publication Abstract to the page
+
*[[Avidin 3D structures|Avidin 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 22035795 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_22035795}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
[[3vhm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VHM OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:022035795</ref><references group="xtra"/>
+
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
-
[[Category: Maki, E.]]
+
[[Category: Large Structures]]
-
[[Category: Matsumura, H.]]
+
[[Category: Maki E]]
-
[[Category: Mori, Y.]]
+
[[Category: Matsumura H]]
-
[[Category: Nagano, T.]]
+
[[Category: Mori Y]]
-
[[Category: Sugiyama, S.]]
+
[[Category: Nagano T]]
-
[[Category: Takahashi, Y.]]
+
[[Category: Sugiyama S]]
-
[[Category: Terai, T.]]
+
[[Category: Takahashi Y]]
-
[[Category: Beta barrel]]
+
[[Category: Terai T]]
-
[[Category: Biotin-binding protein]]
+

Current revision

Crystal structure of NPC-biotin-avidin complex

PDB ID 3vhm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vhm"
Personal tools