2jh3

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[[Image:2jh3.jpg|left|200px]]<br /><applet load="2jh3" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2jh3, resolution 1.90&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF DR2241 FROM DEINOCOCCUS RADIODURANS AT 1.9 A RESOLUTION REVEALS A MULTI-DOMAIN PROTEIN WITH STRUCTURAL SIMILARITY TO CHELATASES BUT ALSO WITH TWO ADDITIONAL NOVEL DOMAINS'''<br />
 
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==Overview==
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==The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 A resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains==
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A unique family of proteins have been identified in the Deinococcus genus, with an N-terminal cobalamin (vitamin B(12)) chelatase domain denoted CbiX, and an additional unique C-terminal domain with unknown function. Here we, report the first crystal structure from this new family of proteins with, the structure of Deinococcus radiodurans protein DR2241. The structure, reveals a multi-domain protein where domains A (residues 1-132) has the, same fold as the small CbiX (CbiX(S)), domains A and B (residues 1-272), follow the chelatase super-family fold and the two additional unique, domains C and D have no structural homologues. Domain D harbours the, sequence motifs CxxC and CxxxC, in which DR2241 gives the first evidence, that these motifs bind a [4Fe-4S] iron-sulphur cluster. In solution there, are indications of multimeric forms, and in the crystallographic, asymmetric unit a tetramer is found where domains C and D are involved in, stabilising the tetrameric assembly.
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<StructureSection load='2jh3' size='340' side='right'caption='[[2jh3]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jh3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JH3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jh3 OCA], [https://pdbe.org/2jh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jh3 RCSB], [https://www.ebi.ac.uk/pdbsum/2jh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jh3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RS86_DEIRA Q9RS86_DEIRA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/2jh3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jh3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A unique family of proteins have been identified in the Deinococcus genus with an N-terminal cobalamin (vitamin B(12)) chelatase domain denoted CbiX and an additional unique C-terminal domain with unknown function. Here we report the first crystal structure from this new family of proteins with the structure of Deinococcus radiodurans protein DR2241. The structure reveals a multi-domain protein where domains A (residues 1-132) has the same fold as the small CbiX (CbiX(S)), domains A and B (residues 1-272) follow the chelatase super-family fold and the two additional unique domains C and D have no structural homologues. Domain D harbours the sequence motifs CxxC and CxxxC, in which DR2241 gives the first evidence that these motifs bind a [4Fe-4S] iron-sulphur cluster. In solution there are indications of multimeric forms, and in the crystallographic asymmetric unit a tetramer is found where domains C and D are involved in stabilising the tetrameric assembly.
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==About this Structure==
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The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 A resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains.,Leiros HK, McSweeney SM J Struct Biol. 2007 Jul;159(1):92-102. Epub 2007 Mar 12. PMID:17448684<ref>PMID:17448684</ref>
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2JH3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Sf4+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JH3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 A resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains., Leiros HK, McSweeney SM, J Struct Biol. 2007 Jul;159(1):92-102. Epub 2007 Mar 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17448684 17448684]
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</div>
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<div class="pdbe-citations 2jh3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Deinococcus radiodurans]]
[[Category: Deinococcus radiodurans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Leiros, H.K.S.]]
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[[Category: Leiros H-KS]]
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[[Category: Mcsweeney, S.M.]]
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[[Category: Mcsweeney SM]]
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[[Category: SF4]]
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[[Category: 4fe-4s iron-sulphur cluster]]
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[[Category: cbix]]
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[[Category: chelatase super-family fold]]
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[[Category: ribosomal protein]]
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[[Category: sad phasing]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:45:44 2008''
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Current revision

The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 A resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains

PDB ID 2jh3

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