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3u5z

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[[Image:3u5z.jpg|left|200px]]
 
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==Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog==
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The line below this paragraph, containing "STRUCTURE_3u5z", creates the "Structure Box" on the page.
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<StructureSection load='3u5z' size='340' side='right'caption='[[3u5z]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3u5z]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. The June 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sliding Clamps'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_6 10.2210/rcsb_pdb/mom_2012_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U5Z FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=08T:[[[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-BIS(OXIDANYL)OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]OXY-OXIDANYL-PHOSPHORYL]OXY-TRIS(FLUORANYL)BERYLLIUM'>08T</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3u5z| PDB=3u5z | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1czd|1czd]], [[1sxj|1sxj]], [[3glf|3glf]], [[1jr3|1jr3]], [[1xxh|1xxh]], [[3u60|3u60]], [[3u61|3u61]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">44, gp44 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 62, gp62 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 45, gp45 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u5z OCA], [https://pdbe.org/3u5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u5z RCSB], [https://www.ebi.ac.uk/pdbsum/3u5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u5z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/DPA44_BPT4 DPA44_BPT4]] Function as a sliding-clamp-loading ATPase enzyme during DNA replication. Required for elongation of primed templates by controlling the polymerase processivity. Possesses DNA-dependent ATPase activity on its own and within the heterodimer gp44/gp62. Progressive binding of ATPs triggers a conformational change in the complex that inhibits ATPase activity.<ref>PMID:16800623</ref> <ref>PMID:18676368</ref> [[https://www.uniprot.org/uniprot/DPA5_BPT4 DPA5_BPT4]] Replisome sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. [[https://www.uniprot.org/uniprot/DPA62_BPT4 DPA62_BPT4]] Function as a sliding-clamp-loading ATPase enzyme during DNA replication. Required for elongation of primed templates by controlling the polymerase processivity. Progressive binding of ATPs triggers a conformational change in the complex that inhibits ATPase activity.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of adenosine triphosphatases (ATPases). We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA. The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit and suggests that clamp closure and ejection of the loader involves disruption of the ATP-dependent match in symmetry. The structures explain how synergy among the loader, the clamp, and DNA can trigger ATP hydrolysis and release of the closed clamp on DNA.
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===Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog===
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How a DNA polymerase clamp loader opens a sliding clamp.,Kelch BA, Makino DL, O'Donnell M, Kuriyan J Science. 2011 Dec 23;334(6063):1675-80. PMID:22194570<ref>PMID:22194570</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_22194570}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3u5z" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22194570 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22194570}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bpt4]]
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[[3u5z]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5Z OCA].
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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==Reference==
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[[Category: Sliding Clamps]]
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<ref group="xtra">PMID:022194570</ref><references group="xtra"/>
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[[Category: Donnell, M O]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Kelch, B A]]
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[[Category: Donnell, M O.]]
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[[Category: Kuriyan, J]]
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[[Category: Kelch, B A.]]
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[[Category: Makino, D L]]
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[[Category: Kuriyan, J.]]
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[[Category: Makino, D L.]]
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[[Category: Aaa+]]
[[Category: Aaa+]]
[[Category: Atp hydrolase]]
[[Category: Atp hydrolase]]

Current revision

Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog

PDB ID 3u5z

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