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2tys

From Proteopedia

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[[Image:2tys.gif|left|200px]]<br />
 
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<applet load="2tys" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2tys, resolution 1.9&Aring;" />
 
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'''CRYSTAL STRUCTURES OF MUTANT (BETAK87T) TRYPTOPHAN SYNTHASE ALPHA2 BETA2 COMPLEX WITH LIGANDS BOUND TO THE ACTIVE SITES OF THE ALPHA AND BETA SUBUNITS REVEAL LIGAND-INDUCED CONFORMATIONAL CHANGES'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURES OF MUTANT (BETAK87T) TRYPTOPHAN SYNTHASE ALPHA2 BETA2 COMPLEX WITH LIGANDS BOUND TO THE ACTIVE SITES OF THE ALPHA AND BETA SUBUNITS REVEAL LIGAND-INDUCED CONFORMATIONAL CHANGES==
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Three-dimensional structures are reported for a mutant (betaK87T), tryptophan synthase alpha2beta2 complex with either the substrate L-serine, (betaK87T-Ser) or product L-tryptophan (betaK87T-Trp) at the active site, of the beta-subunit, in which both amino acids form external aldimines, with the coenzyme, pyridoxal phosphate. We also present structures with, L-serine bound to the beta site and either alpha-glycerol 3-phosphate, (betaK87T-Ser-GP) or indole-3-propanol phosphate (betaK87T-Ser-IPP) bound, to the active site of the alpha-subunit. The results further identify the, substrate and product binding sites in each subunit and provide insight, into conformational changes that occur upon formation of these complexes., The two structures having ligands at the active sites of both alpha- ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9201907 (full description)]]
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<StructureSection load='2tys' size='340' side='right'caption='[[2tys]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2tys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TYS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLT:[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-L-TRYPTOPHANE'>PLT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tys OCA], [https://pdbe.org/2tys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tys RCSB], [https://www.ebi.ac.uk/pdbsum/2tys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tys ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRPA_SALTY TRPA_SALTY] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ty/2tys_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2tys ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2TYS is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA and PLT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Site: S1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TYS OCA]].
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*[[Tryptophan synthase 3D structures|Tryptophan synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of a mutant (betaK87T) tryptophan synthase alpha2beta2 complex with ligands bound to the active sites of the alpha- and beta-subunits reveal ligand-induced conformational changes., Rhee S, Parris KD, Hyde CC, Ahmed SA, Miles EW, Davies DR, Biochemistry. 1997 Jun 24;36(25):7664-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9201907 9201907]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Salmonella typhimurium]]
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[[Category: Ahmed SA]]
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[[Category: Tryptophan synthase]]
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[[Category: Davies DR]]
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[[Category: Ahmed, S.A.]]
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[[Category: Hyde CC]]
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[[Category: Davies, D.R.]]
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[[Category: Miles EW]]
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[[Category: Hyde, C.C.]]
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[[Category: Parris KD]]
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[[Category: Miles, E.W.]]
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[[Category: Rhee S]]
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[[Category: Parris, K.D.]]
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[[Category: Rhee, S.]]
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[[Category: NA]]
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[[Category: PLT]]
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[[Category: carbon-oxygen lyase]]
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[[Category: lyase]]
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[[Category: pyridoxal phosphate]]
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[[Category: tryptophan biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:50:49 2007''
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Current revision

CRYSTAL STRUCTURES OF MUTANT (BETAK87T) TRYPTOPHAN SYNTHASE ALPHA2 BETA2 COMPLEX WITH LIGANDS BOUND TO THE ACTIVE SITES OF THE ALPHA AND BETA SUBUNITS REVEAL LIGAND-INDUCED CONFORMATIONAL CHANGES

PDB ID 2tys

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