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| - | [[Image:3s9v.png|left|200px]] | |
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| - | <!--
| + | ==abietadiene synthase from Abies grandis== |
| - | The line below this paragraph, containing "STRUCTURE_3s9v", creates the "Structure Box" on the page.
| + | <StructureSection load='3s9v' size='340' side='right'caption='[[3s9v]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[3s9v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Abies_grandis Abies grandis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S9V FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s9v OCA], [https://pdbe.org/3s9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s9v RCSB], [https://www.ebi.ac.uk/pdbsum/3s9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s9v ProSAT]</span></td></tr> |
| - | {{STRUCTURE_3s9v| PDB=3s9v | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | ===abietadiene synthase from Abies grandis===
| + | [https://www.uniprot.org/uniprot/TPSDV_ABIGR TPSDV_ABIGR] Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to abietadiene. The copalyl diphosphate (CPP) intermediate diffuses freely between the 2 active sites in the enzyme. Changes in reaction pH, but not salt concentration, influence the relative proportion of the major products of the enzyme, abitadiene, levopimaradiene and neoabitadiene.<ref>PMID:9539701</ref> <ref>PMID:11112547</ref> <ref>PMID:10814381</ref> <ref>PMID:11827528</ref> <ref>PMID:12614165</ref> <ref>PMID:18052062</ref> <ref>PMID:20430888</ref> |
| - | | + | == References == |
| - | | + | <references/> |
| - | ==About this Structure== | + | __TOC__ |
| - | [[3s9v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Abies_grandis Abies grandis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S9V OCA]. | + | </StructureSection> |
| | [[Category: Abies grandis]] | | [[Category: Abies grandis]] |
| - | [[Category: Honzatko, R B.]] | + | [[Category: Large Structures]] |
| - | [[Category: Hoy, J A.]] | + | [[Category: Honzatko RB]] |
| - | [[Category: Mann, F M.]] | + | [[Category: Hoy JA]] |
| - | [[Category: Peters, R J.]] | + | [[Category: Mann FM]] |
| - | [[Category: Zhou, K.]] | + | [[Category: Peters RJ]] |
| - | [[Category: Alpha bundle/barrel]] | + | [[Category: Zhou K]] |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Function
TPSDV_ABIGR Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to abietadiene. The copalyl diphosphate (CPP) intermediate diffuses freely between the 2 active sites in the enzyme. Changes in reaction pH, but not salt concentration, influence the relative proportion of the major products of the enzyme, abitadiene, levopimaradiene and neoabitadiene.[1] [2] [3] [4] [5] [6] [7]
References
- ↑ Bohlmann J, Meyer-Gauen G, Croteau R. Plant terpenoid synthases: molecular biology and phylogenetic analysis. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4126-33. PMID:9539701
- ↑ Peters RJ, Flory JE, Jetter R, Ravn MM, Lee HJ, Coates RM, Croteau RB. Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme. Biochemistry. 2000 Dec 19;39(50):15592-602. PMID:11112547
- ↑ Ravn MM, Coates RM, Flory JE, Peters RJ, Croteau R. Stereochemistry of the cyclization-rearrangement of (+)-copalyl diphosphate to (-)-abietadiene catalyzed by recombinant abietadiene synthase from Abies grandis. Org Lett. 2000 Mar 9;2(5):573-6. PMID:10814381
- ↑ Peters RJ, Croteau RB. Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate. Biochemistry. 2002 Feb 12;41(6):1836-42. PMID:11827528
- ↑ Peters RJ, Carter OA, Zhang Y, Matthews BW, Croteau RB. Bifunctional abietadiene synthase: mutual structural dependence of the active sites for protonation-initiated and ionization-initiated cyclizations. Biochemistry. 2003 Mar 11;42(9):2700-7. PMID:12614165 doi:http://dx.doi.org/10.1021/bi020492n
- ↑ Wilderman PR, Peters RJ. A single residue switch converts abietadiene synthase into a pimaradiene specific cyclase. J Am Chem Soc. 2007 Dec 26;129(51):15736-7. Epub 2007 Dec 1. PMID:18052062 doi:http://dx.doi.org/10.1021/ja074977g
- ↑ Mann FM, Prisic S, Davenport EK, Determan MK, Coates RM, Peters RJ. A single residue switch for Mg(2+)-dependent inhibition characterizes plant class II diterpene cyclases from primary and secondary metabolism. J Biol Chem. 2010 Jul 2;285(27):20558-63. doi: 10.1074/jbc.M110.123307. Epub 2010, Apr 29. PMID:20430888 doi:10.1074/jbc.M110.123307
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