2v43

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[[Image:2v43.jpg|left|200px]]<br /><applet load="2v43" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2v43, resolution 2.37&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RSEB: A SENSOR FOR PERIPLASMIC STRESS RESPONSE IN E. COLI'''<br />
 
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==Overview==
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==Crystal structure of RseB: a sensor for periplasmic stress response in E. coli==
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An elegant network of signal transduction has evolved in the bacterial, cell envelope to respond to environmental stress. It is initiated by, sensing unfavourable and harmful changes in the periplasm. The stress, signal is then transmitted by a controlled degradation of the, transmembrane anti-sigma-factor RseA that leads to the activation of the, alternative sigma factor sigma(E). The periplasmic protein RseB exerts a, crucial role in modulating the stability of RseA. RseB from Escherichia, coli has been crystallized and crystal structures were determined at 2.4 A, and at 2.8 A resolution. The protein forms a homodimer, with the monomer, composed of two domains. The large domain resembles an unclosed, beta-barrel that is structurally remarkably similar to a protein family, capable of binding the lipid anchor of lipoproteins. The small C-terminal, domain, connected to the large domain by a partially unstructured loop, is, responsible for interaction with RseA. On the basis of the structure of, RseB, we suggest that it acts as a sensor of periplasmic stress with a, dual functionality: it detects mislocalized lipoproteins and propagates, the signal to induce the sigma(E)-response.
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<StructureSection load='2v43' size='340' side='right'caption='[[2v43]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2v43]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V43 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYS:CYSTEINE'>CYS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v43 OCA], [https://pdbe.org/2v43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v43 RCSB], [https://www.ebi.ac.uk/pdbsum/2v43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v43 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RSEB_ECOLI RSEB_ECOLI] Seems to modulate the activity of RpoE (sigma-E).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/2v43_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v43 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2V43 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CYS:'>CYS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:CYS+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V43 OCA].
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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__TOC__
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==Reference==
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</StructureSection>
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The Structure of RseB: A Sensor in Periplasmic Stress Response of E. coli., Wollmann P, Zeth K, J Mol Biol. 2007 Jun 19;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17692869 17692869]
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[[Category: Large Structures]]
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[[Category: Escherichia coli]]
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[[Category: Wollmann P]]
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[[Category: Single protein]]
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[[Category: Zeth K]]
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[[Category: Wollmann, P.]]
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[[Category: Zeth, K.]]
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[[Category: CYS]]
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[[Category: lipoprotein binding]]
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[[Category: periplasmic]]
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[[Category: regulator]]
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[[Category: regulatory protein]]
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[[Category: sensor for periplasmic stress]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:49:45 2008''
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Current revision

Crystal structure of RseB: a sensor for periplasmic stress response in E. coli

PDB ID 2v43

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