1oxd

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[[Image:1oxd.png|left|200px]]
 
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==Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins==
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The line below this paragraph, containing "STRUCTURE_1oxd", creates the "Structure Box" on the page.
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<StructureSection load='1oxd' size='340' side='right'caption='[[1oxd]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1oxd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cfp_marker_plasmid_pWM1009 Cfp marker plasmid pWM1009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRF:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CRF</scene></td></tr>
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{{STRUCTURE_1oxd| PDB=1oxd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxd OCA], [https://pdbe.org/1oxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxd RCSB], [https://www.ebi.ac.uk/pdbsum/1oxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxd ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oxd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.
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===Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins===
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Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins.,Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N J Mol Biol. 2003 May 16;328(5):1071-81. PMID:12729742<ref>PMID:12729742</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12729742}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1oxd" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12729742 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12729742}}
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==About this Structure==
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[[1oxd]] is a 1 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1009 Cfp marker plasmid pwm1009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXD OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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*[[Devon McCarthy/Sandbox 1]]
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== References ==
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*[[Green Fluorescent Protein]]
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<references/>
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*[[Green Fluorescent Protein: Research Tool]]
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__TOC__
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*[[Sandbox104]]
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</StructureSection>
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*[[User:Joanne Lau/Sandbox 5]]
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[[Category: Cfp marker plasmid pWM1009]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Azim MK]]
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<ref group="xtra">PMID:012729742</ref><ref group="xtra">PMID:012729750</ref><references group="xtra"/>
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[[Category: Budisa N]]
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[[Category: Cfp marker plasmid pwm1009]]
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[[Category: Holak TA]]
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[[Category: Azim, M K.]]
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[[Category: Huber R]]
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[[Category: Bae, J Hyun.]]
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[[Category: Hyun Bae J]]
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[[Category: Budisa, N.]]
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[[Category: Jung G]]
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[[Category: Holak, T A.]]
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[[Category: Kim JS]]
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[[Category: Huber, R.]]
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[[Category: Moroder L]]
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[[Category: Jung, G.]]
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[[Category: Rubini M]]
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[[Category: Kim, J S.]]
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[[Category: Seifert MH]]
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[[Category: Moroder, L.]]
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[[Category: Wiegand G]]
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[[Category: Rubini, M.]]
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[[Category: Zumbusch A]]
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[[Category: Seifert, M H.]]
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[[Category: Wiegand, G.]]
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[[Category: Zumbusch, A.]]
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[[Category: Amino acid incorporation]]
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[[Category: Chromophore]]
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[[Category: Genetic code]]
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[[Category: Green fluorescent protein]]
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[[Category: Luminescent protein]]
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[[Category: Tryptophan]]
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Current revision

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

PDB ID 1oxd

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