3u01

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[[Image:3u01.png|left|200px]]
 
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==Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution==
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The line below this paragraph, containing "STRUCTURE_3u01", creates the "Structure Box" on the page.
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<StructureSection load='3u01' size='340' side='right'caption='[[3u01]], [[Resolution|resolution]] 1.12&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3u01]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithobates_pipiens Lithobates pipiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U01 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.12&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3u01| PDB=3u01 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u01 OCA], [https://pdbe.org/3u01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u01 RCSB], [https://www.ebi.ac.uk/pdbsum/3u01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u01 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNP30_LITPI RNP30_LITPI] Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of onconase C30A/C75A double mutant has been determined at 1.12A resolution. The structure has high structural homology to other onconase structures. The changes being results of mutation are relatively small, distributed asymmetrically around the two mutated positions, and they are observed not only in the mutation region but expanded to entire molecule. Different conformation of Lys31 side chain that influences the hydrogen bonding network around catalytic triad is probably responsible for lower catalytic efficiency of double mutant. The decrease in thermal stability observed for the onconase variant might be explained by a less dense packing as manifested by the increase of the molecular volume and the solvent accessible surface area. (c) 2013 Wiley Periodicals, Inc. Biopolymers 101: 454-460, 2014.
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===Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution===
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Investigating the effects of double mutation C30A/C75A on onconase structure: Studies at atomic resolution.,Kurpiewska K, Torrent G, Ribo M, Loch JI, Vilanova M, Lewinski K Biopolymers. 2014 May;101(5):454-60. doi: 10.1002/bip.22403. PMID:23996687<ref>PMID:23996687</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3u01]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_pipiens Rana pipiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U01 OCA].
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<div class="pdbe-citations 3u01" style="background-color:#fffaf0;"></div>
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[[Category: Rana pipiens]]
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== References ==
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[[Category: Kurpiewska, K.]]
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<references/>
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[[Category: Lewinski, K.]]
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__TOC__
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[[Category: Loch, J.]]
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</StructureSection>
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[[Category: Ribo, M.]]
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[[Category: Large Structures]]
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[[Category: Torrent, G.]]
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[[Category: Lithobates pipiens]]
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[[Category: Vilanova, M.]]
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[[Category: Kurpiewska K]]
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[[Category: Alpha/beta protein]]
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[[Category: Lewinski K]]
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[[Category: Antitumor protein]]
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[[Category: Loch J]]
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[[Category: Endonuclease]]
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[[Category: Ribo M]]
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[[Category: Hydrolase]]
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[[Category: Torrent G]]
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[[Category: Nuclease]]
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[[Category: Vilanova M]]
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[[Category: Ranpirnase]]
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Current revision

Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution

PDB ID 3u01

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