3ntu

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[[Image:3ntu.png|left|200px]]
 
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==RADA RECOMBINASE D302K MUTANT IN COMPLEX with AMP-PNP==
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The line below this paragraph, containing "STRUCTURE_3ntu", creates the "Structure Box" on the page.
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<StructureSection load='3ntu' size='340' side='right'caption='[[3ntu]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ntu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_voltae_PS Methanococcus voltae PS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NTU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3ntu| PDB=3ntu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ntu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ntu OCA], [https://pdbe.org/3ntu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ntu RCSB], [https://www.ebi.ac.uk/pdbsum/3ntu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ntu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RADA_METVO RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RAD51 mediates homologous recombination (HR) by forming an active DNA nucleoprotein filament (NPF). A conserved aspartate that forms a salt bridge with the ATP gamma-phosphate is found at the nucleotide-binding interface between RAD51 subunits of the NPF known as the ATP cap. The salt bridge accounts for the nonphysiological cation(s) required to fully activate the RAD51 NPF. In contrast, RecA homologs and most RAD51 paralogs contain a conserved lysine at the analogous structural position. We demonstrate that substitution of human RAD51(D316) with lysine [HsRAD51(D316K)] decreases NPF turnover and facilitates considerably improved recombinase functions. Structural analysis shows that archaebacterial Methanococcus voltae RadA(D302K) [MvRAD51(D302K)] and HsRAD51(D316K) form extended active NPFs without salt. These studies suggest that the HsRAD51(D316) salt bridge may function as a conformational sensor that enhances turnover at the expense of recombinase activity.
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===RADA RECOMBINASE D302K MUTANT IN COMPLEX with AMP-PNP===
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The RAD51 ATP cap regulates nucleoprotein filament stability.,Amunugama R, He Y, Willcox S, Forties RA, Shim KS, Bundschuh R, Luo Y, Griffith J, Fishel R J Biol Chem. 2012 Jan 24. PMID:22275364<ref>PMID:22275364</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ntu" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22275364 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22275364}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3ntu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NTU OCA].
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[[Category: Methanococcus voltae PS]]
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[[Category: Luo Y]]
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==Reference==
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<ref group="xtra">PMID:022275364</ref><references group="xtra"/>
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[[Category: Methanococcus voltae]]
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[[Category: Luo, Y.]]
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[[Category: Active conformation]]
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[[Category: Atp complex]]
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[[Category: Atpase]]
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[[Category: Dmc1]]
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[[Category: Rad51]]
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[[Category: Rada]]
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[[Category: Reca]]
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[[Category: Recombinase]]
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[[Category: Recombination]]
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Current revision

RADA RECOMBINASE D302K MUTANT IN COMPLEX with AMP-PNP

PDB ID 3ntu

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