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1of3

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[[Image:1of3.gif|left|200px]]<br />
 
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<applet load="1of3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1of3, resolution 2.00&Aring;" />
 
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'''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE, TMCBM27'''<br />
 
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==Overview==
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==Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27==
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The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5), constitute a carbohydrate binding module (CBM) that has been classified, into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds, tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose, (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal, structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a, TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6, A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for, mannans. In particular, the latter complex, which is the first structure, of a CBM in complex with a branched plant cell wall polysaccharide, illustrates ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12791255 (full description)]]
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<StructureSection load='1of3' size='340' side='right'caption='[[1of3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1of3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of3 OCA], [https://pdbe.org/1of3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of3 RCSB], [https://www.ebi.ac.uk/pdbsum/1of3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RIK9_THEMT Q9RIK9_THEMT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1of3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1of3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OF3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OF3 OCA]].
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12791255 12791255]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Thermotoga maritima]]
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[[Category: Boraston AB]]
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[[Category: Boraston, A.B.]]
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[[Category: Boraston CM]]
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[[Category: Boraston, C.M.]]
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[[Category: Davies GJ]]
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[[Category: Davies, G.J.]]
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[[Category: Nurizzo D]]
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[[Category: Nurizzo, D.]]
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[[Category: Revett TJ]]
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[[Category: Revett, T.J.]]
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[[Category: CA]]
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[[Category: carbohydrate binding module]]
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[[Category: hydrolase]]
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[[Category: mannan binding]]
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[[Category: polysaccharide degradation]]
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[[Category: tmcbm27]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:53:32 2007''
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Current revision

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27

PDB ID 1of3

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