2lo0
From Proteopedia
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| - | [[Image:2lo0.png|left|200px]] | ||
| - | < | + | ==Solution structure of the Get5 carboxyl domain from A. fumigatus== |
| - | + | <StructureSection load='2lo0' size='340' side='right'caption='[[2lo0]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2lo0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LO0 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lo0 OCA], [https://pdbe.org/2lo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lo0 RCSB], [https://www.ebi.ac.uk/pdbsum/2lo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lo0 ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4WE50_ASPFU Q4WE50_ASPFU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex. | ||
| - | + | Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836<ref>PMID:22262836</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | == | + | </div> |
| - | [[ | + | <div class="pdbe-citations 2lo0" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Chartron | + | <references/> |
| - | [[Category: Clemons | + | __TOC__ |
| - | [[Category: Rao | + | </StructureSection> |
| - | [[Category: Vandervelde | + | [[Category: Aspergillus fumigatus Af293]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Chartron JW]] | |
| - | + | [[Category: Clemons Jr WM]] | |
| + | [[Category: Rao M]] | ||
| + | [[Category: Vandervelde DG]] | ||
Current revision
Solution structure of the Get5 carboxyl domain from A. fumigatus
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