3t1h

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[[Image:3t1h.png|left|200px]]
 
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==Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA lysine 3 (tRNALys3) bound to an mRNA with an AAA-codon in the A-site and Paromomycin==
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The line below this paragraph, containing "STRUCTURE_3t1h", creates the "Structure Box" on the page.
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<StructureSection load='3t1h' size='340' side='right'caption='[[3t1h]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3t1h]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12A:2-METHYLTHIO-N6-(AMINOCARBONYL-L-THREONYL)-ADENOSINE-5-MONOPHOSPHATE'>12A</scene>, <scene name='pdbligand=70U:5-(O-METHYLACETO)-2-THIO-2-DEOXY-URIDINE-5-MONOPHOSPHATE'>70U</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3t1h| PDB=3t1h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1h OCA], [https://pdbe.org/3t1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1h RCSB], [https://www.ebi.ac.uk/pdbsum/3t1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human tRNA(Lys3)(UUU) (htRNA(Lys3)(UUU)) decodes the lysine codons AAA and AAG during translation and also plays a crucial role as the primer for HIV-1 (human immunodeficiency virus type 1) reverse transcription. The posttranscriptional modifications 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U(34)), 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A(37)), and pseudouridine (Psi(39)) in the tRNA's anticodon domain are critical for ribosomal binding and HIV-1 reverse transcription. To understand the importance of modified nucleoside contributions, we determined the structure and function of this tRNA's anticodon stem and loop (ASL) domain with these modifications at positions 34, 37, and 39, respectively (hASL(Lys3)(UUU)-mcm(5)s(2)U(34);ms(2)t(6)A(37);Psi(39)). Ribosome binding assays in vitro revealed that the hASL(Lys3)(UUU)-mcm(5)s(2)U(34);ms(2)t(6)A(37);Psi(39) bound AAA and AAG codons, whereas binding of the unmodified ASL(Lys3)(UUU) was barely detectable. The UV hyperchromicity, the circular dichroism, and the structural analyses indicated that Psi(39) enhanced the thermodynamic stability of the ASL through base stacking while ms(2)t(6)A(37) restrained the anticodon to adopt an open loop conformation that is required for ribosomal binding. The NMR-restrained molecular-dynamics-derived solution structure revealed that the modifications provided an open, ordered loop for codon binding. The crystal structures of the hASL(Lys3)(UUU)-mcm(5)s(2)U(34);ms(2)t(6)A(37);Psi(39) bound to the 30S ribosomal subunit with each codon in the A site showed that the modified nucleotides mcm(5)s(2)U(34) and ms(2)t(6)A(37) participate in the stability of the anticodon-codon interaction. Importantly, the mcm(5)s(2)U(34).G(3) wobble base pair is in the Watson-Crick geometry, requiring unusual hydrogen bonding to G in which mcm(5)s(2)U(34) must shift from the keto to the enol form. The results unambiguously demonstrate that modifications pre-structure the anticodon as a key prerequisite for efficient and accurate recognition of cognate and wobble codons.
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===Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA lysine 3 (tRNALys3) bound to an mRNA with an AAA-codon in the A-site and Paromomycin===
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Human tRNA(Lys3)(UUU) Is Pre-Structured by Natural Modifications for Cognate and Wobble Codon Binding through Keto-Enol Tautomerism.,Vendeix FA, Murphy FV 4th, Cantara WA, Leszczynska G, Gustilo EM, Sproat B, Malkiewicz A, Agris PF J Mol Biol. 2011 Dec 29. PMID:22227389<ref>PMID:22227389</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3t1h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22227389}}, adds the Publication Abstract to the page
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22227389 is the PubMed ID number.
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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{{ABSTRACT_PUBMED_22227389}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3t1h]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1H OCA].
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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[[Category: Thermus thermophilus HB27]]
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<ref group="xtra">PMID:022227389</ref><references group="xtra"/>
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[[Category: Thermus thermophilus HB8]]
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[[Category: Thermus thermophilus]]
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[[Category: Agris PF]]
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[[Category: Agris, P F.]]
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[[Category: Cantara W]]
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[[Category: Cantara, W.]]
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[[Category: Gustilo EM]]
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[[Category: Gustilo, E M.]]
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[[Category: Leszczynska G]]
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[[Category: Leszczynska, G.]]
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[[Category: Malkiewicz AAP]]
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[[Category: Malkiewicz, A A.P.]]
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[[Category: Murphy FV]]
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[[Category: Murphy, F V.]]
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[[Category: Sproat B]]
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[[Category: Sproat, B.]]
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[[Category: Vendeix FAP]]
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[[Category: Vendeix, F A.P.]]
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[[Category: Protein synthesis]]
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[[Category: Ribosome-antibiotic complex]]
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[[Category: Trna]]
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Current revision

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA lysine 3 (tRNALys3) bound to an mRNA with an AAA-codon in the A-site and Paromomycin

PDB ID 3t1h

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