3uq8

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[[Image:3uq8.png|left|200px]]
 
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==Structure of adenylation domain of Haemophilus influenzae DNA ligases bound to NAD+ in adenylated state.==
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The line below this paragraph, containing "STRUCTURE_3uq8", creates the "Structure Box" on the page.
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<StructureSection load='3uq8' size='340' side='right'caption='[[3uq8]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3uq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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{{STRUCTURE_3uq8| PDB=3uq8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq8 OCA], [https://pdbe.org/3uq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq8 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNLJ_HAEIN DNLJ_HAEIN] DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NAD+-dependent DNA ligases (LigA) are essential bacterial enzymes that catalyze phosphodiester bond formation during DNA replication and repair processes. Phosphodiester bond formation proceeds through a well-conserved 3-step reaction mechanism. In the first step, the LigA adenylation domain interacts with NAD+ to form a covalent enzyme-AMP. Although it is well-established that the specificity for binding of NAD+ resides within the adenylation domain, the precise recognition elements for the initial binding event remain unclear. We report here the structure of the adenylation domain from Haemophilus influenzae LigA. This structure is a first snapshot of a LigA-AMP intermediate with NAD+ bound to domain 1a in its open conformation. The binding affinities of NAD+ for adenylated and non-adenylated forms of the H. influenzae LigA adenylation domain were measured using isothermal titration calorimetry. NAD+ was only observed to bind to domain 1a and not domain 1b. The combined crystalographic and NAD+-binding data suggest that the initial recognition of NAD+ is via the NMN binding region in domain 1a of LigA.
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===Structure of adenylation domain of Haemophilus influenzae DNA ligases bound to NAD+ in adenylated state.===
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Structure guided understanding of NAD+ recognition in bacterial DNA ligases.,Lahiri SD, Mills S, Gu RF, Gao N, Walkup G, Karantzeni I ACS Chem Biol. 2012 Jan 9. PMID:22230472<ref>PMID:22230472</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3uq8" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[DNA ligase 3D structures|DNA ligase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22230472 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22230472}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Haemophilus influenzae Rd KW20]]
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[[3uq8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ8 OCA].
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[[Category: Large Structures]]
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[[Category: Lahiri SD]]
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==Reference==
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<ref group="xtra">PMID:022230472</ref><references group="xtra"/>
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[[Category: Haemophilus influenzae]]
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[[Category: Lahiri, S D.]]
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[[Category: Adenylated protein]]
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[[Category: Adenylation]]
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[[Category: Atp-grasp]]
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[[Category: Ligase]]
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[[Category: Rossman fold]]
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Current revision

Structure of adenylation domain of Haemophilus influenzae DNA ligases bound to NAD+ in adenylated state.

PDB ID 3uq8

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