4ads

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[[Image:4ads.png|left|200px]]
 
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==Crystal structure of plasmodial PLP synthase complex==
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The line below this paragraph, containing "STRUCTURE_4ads", creates the "Structure Box" on the page.
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<StructureSection load='4ads' size='340' side='right'caption='[[4ads]], [[Resolution|resolution]] 3.61&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4ads]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_berghei Plasmodium berghei] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_4ads| PDB=4ads | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ads FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ads OCA], [https://pdbe.org/4ads PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ads RCSB], [https://www.ebi.ac.uk/pdbsum/4ads PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ads ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDX2_PLAF7 PDX2_PLAF7] Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of Pdx1.<ref>PMID:15590634</ref> <ref>PMID:16339145</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Biosynthesis of vitamins is fundamental to malaria parasites. Plasmodia synthesize the active form of vitamin B(6) (pyridoxal 5'-phosphate, PLP) using a PLP synthase complex. The EM analysis shown here reveals a random association pattern of up to 12 Pdx2 glutaminase subunits to the dodecameric Pdx1 core complex. Interestingly, Plasmodium falciparum PLP synthase organizes in fibers. The crystal structure shows differences in complex formation to bacterial orthologs as interface variations. Alternative positioning of an alpha helix distinguishes an open conformation from a closed state when the enzyme binds substrate. The pentose substrate is covalently attached through its C1 and forms a Schiff base with Lys84. Ammonia transfer between Pdx2 glutaminase and Pdx1 active sites is regulated by a transient tunnel. The mutagenesis analysis allows defining the requirement for conservation of critical methionines, whereas there is also plasticity in ammonia tunnel construction as seen from comparison across different species.
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===Crystal structure of plasmodial PLP synthase complex===
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Assembly of the Eukaryotic PLP-Synthase Complex from Plasmodium and Activation of the Pdx1 Enzyme.,Guedez G, Hipp K, Windeisen V, Derrer B, Gengenbacher M, Bottcher B, Sinning I, Kappes B, Tews I Structure. 2012 Jan 11;20(1):172-84. PMID:22244765<ref>PMID:22244765</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ads" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22244765 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22244765}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[4ads]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_berghei Plasmodium berghei] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADS OCA].
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==Reference==
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<ref group="xtra">PMID:022244765</ref><references group="xtra"/>
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[[Category: Plasmodium berghei]]
[[Category: Plasmodium berghei]]
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[[Category: Plasmodium falciparum]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Guedez, G.]]
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[[Category: Guedez G]]
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[[Category: Sinning, I.]]
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[[Category: Sinning I]]
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[[Category: Tews, I.]]
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[[Category: Tews I]]
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[[Category: Pyridoxal 5-phosphate biosynthesis]]
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[[Category: Transferase-transferase complex]]
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Current revision

Crystal structure of plasmodial PLP synthase complex

PDB ID 4ads

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