3euk

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[[Image:3euk.png|left|200px]]
 
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==Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, asymmetric dimer==
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The line below this paragraph, containing "STRUCTURE_3euk", creates the "Structure Box" on the page.
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<StructureSection load='3euk' size='340' side='right'caption='[[3euk]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3euk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi] and [https://en.wikipedia.org/wiki/Haemophilus_ducreyi_35000HP Haemophilus ducreyi 35000HP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EUK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_3euk| PDB=3euk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3euk OCA], [https://pdbe.org/3euk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3euk RCSB], [https://www.ebi.ac.uk/pdbsum/3euk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3euk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUKB_HAEDU MUKB_HAEDU] Plays a central role in chromosome condensation, segregation and cell cycle progression. Functions as a homodimer, which is essential for chromosome partition. Involved in negative DNA supercoiling in vivo, and by this means organize and compact chromosomes. May achieve or facilitate chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/3euk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3euk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Condensins are key mediators of chromosome condensation across organisms. Like other condensins, the bacterial MukBEF condensin complex consists of an SMC family protein dimer containing two ATPase head domains, MukB, and two interacting subunits, MukE and MukF. We report complete structural views of the intersubunit interactions of this condensin along with ensuing studies that reveal a role for the ATPase activity of MukB. MukE and MukF together form an elongated dimeric frame, and MukF's C-terminal winged-helix domains (C-WHDs) bind MukB heads to constitute closed ring-like structures. Surprisingly, one of the two bound C-WHDs is forced to detach upon ATP-mediated engagement of MukB heads. This detachment reaction depends on the linker segment preceding the C-WHD, and mutations on the linker restrict cell growth. Thus ATP-dependent transient disruption of the MukB-MukF interaction, which creates openings in condensin ring structures, is likely to be a critical feature of the functional mechanism of condensins.
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===Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, asymmetric dimer===
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Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions.,Woo JS, Lim JH, Shin HC, Suh MK, Ku B, Lee KH, Joo K, Robinson H, Lee J, Park SY, Ha NC, Oh BH Cell. 2009 Jan 9;136(1):85-96. PMID:19135891<ref>PMID:19135891</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3euk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19135891}}, adds the Publication Abstract to the page
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*[[Condensin|Condensin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19135891 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19135891}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3euk]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi] and [http://en.wikipedia.org/wiki/Haemophilus_ducreyi,_synthetic Haemophilus ducreyi, synthetic]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUK OCA].
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[[Category: Lim JH]]
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[[Category: Oh BH]]
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==Reference==
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[[Category: Shin HC]]
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<ref group="xtra">PMID:019135891</ref><references group="xtra"/>
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[[Category: Woo JS]]
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[[Category: Haemophilus ducreyi]]
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[[Category: Haemophilus ducreyi, synthetic]]
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[[Category: Lim, J H.]]
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[[Category: Oh, B H.]]
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[[Category: Shin, H C.]]
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[[Category: Woo, J S.]]
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[[Category: Abc-type atpase]]
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[[Category: Atp-binding]]
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[[Category: Cell cycle]]
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[[Category: Cell division]]
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[[Category: Chromosome condensation]]
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[[Category: Chromosome partition]]
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[[Category: Condensin]]
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[[Category: Dna condensation]]
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[[Category: Dna-binding]]
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[[Category: Mukb]]
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[[Category: Muke]]
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[[Category: Mukf]]
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[[Category: Non-smc subunit]]
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[[Category: Nucleotide-binding]]
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[[Category: Smc]]
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[[Category: Whd]]
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Current revision

Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, asymmetric dimer

PDB ID 3euk

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