2lox

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[[Image:2lox.jpg|left|200px]]
 
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==NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2==
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The line below this paragraph, containing "STRUCTURE_2lox", creates the "Structure Box" on the page.
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<StructureSection load='2lox' size='340' side='right'caption='[[2lox]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2lox]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lox OCA], [https://pdbe.org/2lox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lox RCSB], [https://www.ebi.ac.uk/pdbsum/2lox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lox ProSAT]</span></td></tr>
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{{STRUCTURE_2lox| PDB=2lox | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TFB1_YEAST TFB1_YEAST] Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.<ref>PMID:7961739</ref> <ref>PMID:8631896</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The general transcription factor IIH (TFIIH) plays crucial roles in transcription as part of the pre-initiation complex (PIC) and in DNA repair as part of the nucleotide excision repair (NER) machinery. During NER, TFIIH recruits the 3'-endonuclease Rad2 to damaged DNA. In this manuscript, we functionally and structurally characterized the interaction between the Tfb1 subunit of TFIIH and Rad2. We show that deletion of either the PH domain of Tfb1 (Tfb1PH) or several segments of the Rad2 spacer region yield yeast with enhanced sensitivity to UV irradiation. Isothermal titration calorimetry studies demonstrate that two acidic segments of the Rad2 spacer bind to Tfb1PH with nanomolar affinity. Structure determination of a Rad2-Tfb1PH complex indicates that Rad2 binds to TFIIH using a similar motif as TFIIEalpha uses to bind TFIIH in the PIC. Together, these results provide a mechanistic bridge between the role of TFIIH in transcription and DNA repair.
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===NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2===
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Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2.,Lafrance-Vanasse J, Arseneault G, Cappadocia L, Chen HT, Legault P, Omichinski JG Nucleic Acids Res. 2012 Feb 28. PMID:22373916<ref>PMID:22373916</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2lox]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOX OCA].
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<div class="pdbe-citations 2lox" style="background-color:#fffaf0;"></div>
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[[Category: Saccharomyces cerevisiae]]
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== References ==
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[[Category: Lafrance-Vanasse, J.]]
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<references/>
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[[Category: Legault, P.]]
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__TOC__
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[[Category: Omichinski, J.]]
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</StructureSection>
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[[Category: Transcription-hydrolase complex]]
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Lafrance-Vanasse J]]
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[[Category: Legault P]]
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[[Category: Omichinski J]]

Current revision

NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2

PDB ID 2lox

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