2hqk

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[[Image:2hqk.png|left|200px]]
 
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==Crystal structure of a monomeric cyan fluorescent protein derived from Clavularia==
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The line below this paragraph, containing "STRUCTURE_2hqk", creates the "Structure Box" on the page.
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<StructureSection load='2hqk' size='340' side='right'caption='[[2hqk]], [[Resolution|resolution]] 1.19&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.19&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2hqk| PDB=2hqk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqk OCA], [https://pdbe.org/2hqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqk RCSB], [https://www.ebi.ac.uk/pdbsum/2hqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFPL_CLASP GFPL_CLASP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The arsenal of engineered variants of the GFP [green FP (fluorescent protein)] from Aequorea jellyfish provides researchers with a powerful set of tools for use in biochemical and cell biology research. The recent discovery of diverse FPs in Anthozoa coral species has provided protein engineers with an abundance of alternative progenitor FPs from which improved variants that complement or supersede existing Aequorea GFP variants could be derived. Here, we report the engineering of the first monomeric version of the tetrameric CFP (cyan FP) cFP484 from Clavularia coral. Starting from a designed synthetic gene library with mammalian codon preferences, we identified dimeric cFP484 variants with fluorescent brightness significantly greater than the wild-type protein. Following incorporation of dimer-breaking mutations and extensive directed evolution with selection for blue-shifted emission, high fluorescent brightness and photostability, we arrived at an optimized variant that we have named mTFP1 [monomeric TFP1 (teal FP 1)]. The new mTFP1 is one of the brightest and most photostable FPs reported to date. In addition, the fluorescence is insensitive to physiologically relevant pH changes and the fluorescence lifetime decay is best fitted as a single exponential. The 1.19 A crystal structure (1 A=0.1 nm) of mTFP1 confirms the monomeric structure and reveals an unusually distorted chromophore conformation. As we experimentally demonstrate, the high quantum yield of mTFP1 (0.85) makes it particularly suitable as a replacement for ECFP (enhanced CFP) or Cerulean as a FRET (fluorescence resonance energy transfer) donor to either a yellow or orange FP acceptor.
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===Crystal structure of a monomeric cyan fluorescent protein derived from Clavularia===
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Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.,Ai HW, Henderson JN, Remington SJ, Campbell RE Biochem J. 2006 Dec 15;400(3):531-40. PMID:16859491<ref>PMID:16859491</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16859491}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2hqk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16859491 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16859491}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Clavularia sp]]
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[[2hqk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQK OCA].
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[[Category: Large Structures]]
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[[Category: Ai H]]
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==Reference==
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[[Category: Campbell RE]]
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<ref group="xtra">PMID:016859491</ref><references group="xtra"/>
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[[Category: Henderson JN]]
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[[Category: Clavularia sp.]]
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[[Category: Remington SJ]]
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[[Category: Ai, H.]]
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[[Category: Campbell, R E.]]
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[[Category: Henderson, J N.]]
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[[Category: Remington, S J.]]
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[[Category: 11-stranded beta barrel]]
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[[Category: Luminescent protein]]
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Current revision

Crystal structure of a monomeric cyan fluorescent protein derived from Clavularia

PDB ID 2hqk

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