2e3m

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[[Image:2e3m.jpg|left|200px]]<br /><applet load="2e3m" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2e3m, resolution 2.20&Aring;" />
 
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'''Crystal structure of CERT START domain'''<br />
 
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==Overview==
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==Crystal structure of CERT START domain==
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In mammalian cells, ceramide is synthesized in the endoplasmic reticulum, and transferred to the Golgi apparatus for conversion to sphingomyelin., Ceramide transport occurs in a nonvesicular manner and is mediated by, CERT, a cytosolic 68-kDa protein with a C-terminal steroidogenic acute, regulatory protein-related lipid transfer (START) domain. The CERT START, domain efficiently transfers natural D-erythro-C16-ceramide, but not, lipids with longer (C20) amide-acyl chains. The molecular mechanisms of, ceramide specificity, both stereo-specific recognition and length limit, are not well understood. Here we report the crystal structures of the CERT, START domain in its apo-form and in complex with ceramides having, different acyl chain lengths. In these complex structures, one ceramide, molecule is buried in a long amphiphilic cavity. At the far end of the, cavity, the amide and hydroxyl groups of ceramide form a hydrogen bond, network with specific amino acid residues that play key roles in, stereo-specific ceramide recognition. At the head of the ceramide, molecule, there is no extra space to accommodate additional bulky groups., The two aliphatic chains of ceramide are surrounded by the hydrophobic, wall of the cavity, whose size and shape dictate the length limit for, cognate ceramides. Furthermore, local high-crystallographic B-factors, suggest that the alpha-3 and the Omega1 loop might work as a gate to, incorporate the ceramide into the cavity. Thus, the structures demonstrate, the structural basis for the mechanism by which CERT can distinguish, ceramide from other lipid types yet still recognize multiple species of, ceramides.
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<StructureSection load='2e3m' size='340' side='right'caption='[[2e3m]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2e3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E3M FirstGlance]. <br>
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2E3M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3M OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3m OCA], [https://pdbe.org/2e3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e3m RCSB], [https://www.ebi.ac.uk/pdbsum/2e3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3m ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide., Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R, Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):488-93. Epub 2008 Jan 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184806 18184806]
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== Disease ==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
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== Function ==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.<ref>PMID:14685229</ref> <ref>PMID:17591919</ref> <ref>PMID:18184806</ref> <ref>PMID:20036255</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/2e3m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e3m ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hanada, K.]]
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[[Category: Hanada K]]
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[[Category: Kato, R.]]
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[[Category: Kato R]]
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[[Category: Kudo, N.]]
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[[Category: Kudo N]]
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[[Category: Kumagai, K.]]
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[[Category: Kumagai K]]
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[[Category: Nishijima, M.]]
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[[Category: Nishijima M]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki S]]
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[[Category: ceramide transfer]]
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[[Category: cert]]
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[[Category: lipid transfer protein]]
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[[Category: lipid transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:17:01 2008''
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Current revision

Crystal structure of CERT START domain

PDB ID 2e3m

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