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2eak

From Proteopedia

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[[Image:2eak.png|left|200px]]
 
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==Crystal structure of human galectin-9 N-terminal CRD in complex with lactose==
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The line below this paragraph, containing "STRUCTURE_2eak", creates the "Structure Box" on the page.
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<StructureSection load='2eak' size='340' side='right'caption='[[2eak]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2eak]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EAK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTV:(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTV</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
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-->
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2eag|2eag]], [[2eah|2eah]], [[2eai|2eai]], [[2eaj|2eaj]], [[2eal|2eal]]</div></td></tr>
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{{STRUCTURE_2eak| PDB=2eak | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eak OCA], [https://pdbe.org/2eak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eak RCSB], [https://www.ebi.ac.uk/pdbsum/2eak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eak ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/LEG9_HUMAN LEG9_HUMAN]] Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.<ref>PMID:16286920</ref> <ref>PMID:18005988</ref> <ref>PMID:18977853</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2eak_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eak ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Galectins are a family of beta-galactoside-binding lectins that contain a conserved carbohydrate recognition domain (CRD). They exhibit high affinities for small beta-galactosides as well as variable binding specificities for complex glycoconjugates. Structural and biochemical analyses of the mechanism governing specific carbohydrate recognition provide a useful template to elucidate the function of these proteins. Here we report the crystal structures of the human galectin-9 N-terminal CRD (NCRD) in the presence of lactose and Forssman pentasaccharide. Mouse galectin-9 NCRD, the structure of which was previously solved by our group, forms a non-canonical dimer in both the crystal state and in solution. Human galectin-9 NCRD, however, exists as a monomer in crystals, despite a high sequence identity to the mouse homologue. Comparative frontal affinity chromatography analysis of the mouse and human galectin-9 NCRDs revealed different carbohydrate binding specificities, with disparate affinities for complex glycoconjugates. Human galectin-9 NCRD exhibited a high affinity for Forssman pentasaccharide; the association constant for mouse galectin-9 NCRD was 100-fold less than that observed for the human protein. The combination of structural data with mutational studies demonstrated that non-conserved amino acid residues on the concave surface were important for determination of target specificities. The human galectin-9 NCRD exhibited greater inhibition of cell proliferation than the mouse NCRD. We discuss the biochemical and structural differences between highly homologous proteins from different species.
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===Crystal structure of human galectin-9 N-terminal CRD in complex with lactose===
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Structural analysis of the human galectin-9 N-terminal carbohydrate recognition domain reveals unexpected properties that differ from the mouse orthologue.,Nagae M, Nishi N, Nakamura-Tsuruta S, Hirabayashi J, Wakatsuki S, Kato R J Mol Biol. 2008 Jan 4;375(1):119-35. Epub 2007 Sep 26. PMID:18005988<ref>PMID:18005988</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2eak" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18005988}}, adds the Publication Abstract to the page
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*[[Galectin 3D structures|Galectin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18005988 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18005988}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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[[2eak]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAK OCA].
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[[Category: Large Structures]]
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[[Category: Hirabayashi, J]]
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==Reference==
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[[Category: Kato, R]]
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<ref group="xtra">PMID:018005988</ref><references group="xtra"/>
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[[Category: Nagae, M]]
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[[Category: Homo sapiens]]
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[[Category: Nakamura, T]]
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[[Category: Hirabayashi, J.]]
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[[Category: Nakamura-Tsuruta, S]]
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[[Category: Kato, R.]]
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[[Category: Nishi, N]]
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[[Category: Nagae, M.]]
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[[Category: Wakatsuki, S]]
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[[Category: Nakamura, T.]]
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[[Category: Nakamura-Tsuruta, S.]]
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[[Category: Nishi, N.]]
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[[Category: Wakatsuki, S.]]
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[[Category: Beta sandwich]]
[[Category: Beta sandwich]]
[[Category: Carbohydrate binding protein]]
[[Category: Carbohydrate binding protein]]
[[Category: Galectin]]
[[Category: Galectin]]
[[Category: Sugar binding protein]]
[[Category: Sugar binding protein]]

Current revision

Crystal structure of human galectin-9 N-terminal CRD in complex with lactose

PDB ID 2eak

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