3qr8

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[[Image:3qr8.png|left|200px]]
 
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==Crystal structure of the bacteriophage P2 membrane-piercing protein gpV==
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The line below this paragraph, containing "STRUCTURE_3qr8", creates the "Structure Box" on the page.
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<StructureSection load='3qr8' size='340' side='right'caption='[[3qr8]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3qr8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P2 Escherichia virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QR8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3qr8| PDB=3qr8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qr8 OCA], [https://pdbe.org/3qr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qr8 RCSB], [https://www.ebi.ac.uk/pdbsum/3qr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qr8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPIKE_BPP2 SPIKE_BPP2] Forms the small spikes on the baseplate that plug the end of the tube before DNA ejection and form a channel perforating the host membrane during ejection. Involved in baseplate assembly.<ref>PMID:21821878</ref> <ref>PMID:22325780</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophages with contractile tails and the bacterial type VI secretion system have been proposed to use a special protein to create an opening in the host cell membrane during infection. These proteins have a modular architecture but invariably contain an oligonucleotide/oligosaccharide-binding (OB-fold) domain and a long beta-helical C-terminal domain, which initiates the contact with the host cell membrane. Using X-ray crystallography and electron microscopy, we report the atomic structure of the membrane-piercing proteins from bacteriophages P2 and varphi92 and identify the residues that constitute the membrane-attacking apex. Both proteins form compact spikes with a approximately 10A diameter tip that is stabilized by a centrally positioned iron ion bound by six histidine residues. The accumulated data strongly suggest that, in the process of membrane penetration, the spikes are translocated through the lipid bilayer without undergoing major unfolding.
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===Crystal structure of the bacteriophage P2 membrane-piercing protein gpV===
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Phage pierces the host cell membrane with the iron-loaded spike.,Browning C, Shneider MM, Bowman VD, Schwarzer D, Leiman PG Structure. 2012 Feb 8;20(2):326-39. PMID:22325780<ref>PMID:22325780</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_22325780}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3qr8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22325780 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22325780}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus P2]]
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[[3qr8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p2 Enterobacteria phage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QR8 OCA].
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[[Category: Large Structures]]
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[[Category: Browning C]]
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==Reference==
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[[Category: Leiman PG]]
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<ref group="xtra">PMID:022325780</ref><references group="xtra"/>
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[[Category: Shneider M]]
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[[Category: Enterobacteria phage p2]]
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[[Category: Browning, C.]]
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[[Category: Leiman, P G.]]
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[[Category: Shneider, M.]]
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[[Category: Beta-helix]]
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[[Category: Cell membrane piercing]]
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[[Category: Iron-binding]]
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[[Category: Ob-fold]]
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[[Category: Phage baseplate]]
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[[Category: Tail spike]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of the bacteriophage P2 membrane-piercing protein gpV

PDB ID 3qr8

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