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1a66

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[[Image:1a66.jpg|left|200px]]<br /><applet load="1a66" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a66" />
 
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'''SOLUTION NMR STRUCTURE OF THE CORE NFATC1/DNA COMPLEX, 18 STRUCTURES'''<br />
 
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==Overview==
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==SOLUTION NMR STRUCTURE OF THE CORE NFATC1/DNA COMPLEX, 18 STRUCTURES==
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The nuclear factor of the activated T cell (NFAT) family of transcription, factors regulates cytokine gene expression by binding to the, promoter/enhancer regions of antigen-responsive genes, usually in, cooperation with heterologous DNA-binding partners. Here we report the, solution structure of the binary complex formed between the core, DNA-binding domain of human NFATC1 and the ARRE2 DNA site from the, interleukin-2 promoter. The structure reveals that DNA binding induces the, folding of key structural elements that are required for both, sequence-specific recognition and the establishment of cooperative, protein-protein contacts. The orientation of the NFAT DNA-binding domain, observed in the binary NFATC1-DBD*/ DNA complex is distinct from that seen, in the ternary NFATC2/AP-1/DNA complex, suggesting that the domain, reorients upon formation of a cooperative transcriptional complex.
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<StructureSection load='1a66' size='340' side='right'caption='[[1a66]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a66]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A66 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a66 OCA], [https://pdbe.org/1a66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a66 RCSB], [https://www.ebi.ac.uk/pdbsum/1a66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a66 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NFAC1_HUMAN NFAC1_HUMAN] Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a66_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a66 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The nuclear factor of the activated T cell (NFAT) family of transcription factors regulates cytokine gene expression by binding to the promoter/enhancer regions of antigen-responsive genes, usually in cooperation with heterologous DNA-binding partners. Here we report the solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the ARRE2 DNA site from the interleukin-2 promoter. The structure reveals that DNA binding induces the folding of key structural elements that are required for both sequence-specific recognition and the establishment of cooperative protein-protein contacts. The orientation of the NFAT DNA-binding domain observed in the binary NFATC1-DBD*/ DNA complex is distinct from that seen in the ternary NFATC2/AP-1/DNA complex, suggesting that the domain reorients upon formation of a cooperative transcriptional complex.
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==About this Structure==
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Solution structure of the core NFATC1/DNA complex.,Zhou P, Sun LJ, Dotsch V, Wagner G, Verdine GL Cell. 1998 Mar 6;92(5):687-96. PMID:9506523<ref>PMID:9506523</ref>
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1A66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A66 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the core NFATC1/DNA complex., Zhou P, Sun LJ, Dotsch V, Wagner G, Verdine GL, Cell. 1998 Mar 6;92(5):687-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9506523 9506523]
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</div>
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<div class="pdbe-citations 1a66" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Doetsch, V.]]
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[[Category: Doetsch V]]
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[[Category: Sun, L.J.]]
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[[Category: Sun LJ]]
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[[Category: Verdine, G.L.]]
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[[Category: Verdine GL]]
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[[Category: Wagner, G.]]
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[[Category: Wagner G]]
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[[Category: Zhou, P.]]
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[[Category: Zhou P]]
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[[Category: arre2]]
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[[Category: binary]]
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[[Category: binary complex]]
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[[Category: complex]]
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[[Category: enhanceosome]]
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[[Category: il-2]]
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[[Category: nfat]]
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[[Category: nfat/dna]]
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[[Category: nfat2]]
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[[Category: nfatc]]
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[[Category: nfatc1]]
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[[Category: nfatc1/dna]]
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[[Category: nmr]]
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[[Category: rel]]
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[[Category: transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:28:21 2008''
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Current revision

SOLUTION NMR STRUCTURE OF THE CORE NFATC1/DNA COMPLEX, 18 STRUCTURES

PDB ID 1a66

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