3nbe

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[[Image:3nbe.png|left|200px]]
 
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==Clitocybe nebularis ricin B-like lectin (CNL) in complex with N,N'-diacetyllactosediamine==
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The line below this paragraph, containing "STRUCTURE_3nbe", creates the "Structure Box" on the page.
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<StructureSection load='3nbe' size='340' side='right'caption='[[3nbe]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nbe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clitocybe_nebularis Clitocybe nebularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NBE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PRD_900075:N,N-diacetyllactosediamine'>PRD_900075</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Z3Q:2-AZIDOETHYL+2-(ACETYLAMINO)-2-DEOXY-BETA-D-GLUCOPYRANOSIDE'>Z3Q</scene></td></tr>
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{{STRUCTURE_3nbe| PDB=3nbe | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nbe OCA], [https://pdbe.org/3nbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nbe RCSB], [https://www.ebi.ac.uk/pdbsum/3nbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nbe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CNL_CLINE CNL_CLINE] Lectin specific for terminal, non-reducing N-acetylgalactosamine (Gal-NAc)-containing carbohydrates including N,N'-diacetyllactosediamine/LDN (GalNAcbeta1-4GlcNAc, LacdiNAc). Specific also for carbohydrates containing N-acetylglucosamine (-GlcNAc) or N-acetyllactosamine (-Galbeta1-4GlcNAc) at the reducing end. Agglutinates human blood group A, AB, B and O erythrocytes with a strong preference for group A. Agglutinates bovine erythrocytes with a very low specificity (PubMed:19100814, PubMed:22298779). Binds carbohydrates bivalently, which is required for its biological activity (PubMed:22298779). Exhibits insecticidal activity against the fruit fly D.melanogaster, mosquito A.aegypti, and amoebozoa A.castellanii. Has anti-nutritional activity against Colorado potato beetle L.decemlineata, and against worm C.elegans (PubMed:21556921, PubMed:21486374). Has antiproliferative activity against human leukemic T-cells (PubMed:19100814). Has an immunostimulatory effect on human antigen-presenting dendritic cells, which are subsequently able to induce efficient T-cell immune responses (PubMed:22044067).<ref>PMID:19100814</ref> <ref>PMID:21486374</ref> <ref>PMID:21556921</ref> <ref>PMID:22044067</ref> <ref>PMID:22298779</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lectins are carbohydrate-binding proteins that exert their biological activity by binding to specific cell glycoreceptors. We have expressed CNL, a ricin B-like lectin from the basidiomycete Clitocybe nebularis, in Escherichia coli. The recombinant lectin, rCNL, agglutinates human blood group A erythrocytes and is specific for the unique glycan N,N'-diacetyllactosediamine (GalNAcbeta1-4GlcNAc, LacdiNAc, LDN) as demonstrated by glycan microarray analysis. We here describe the crystal structures of rCNL in complex with lactose and LDN, defining its interactions with the sugars. CNL is a homodimeric lectin, each of whose monomers comprises a single ricin B lectin domain with its beta-trefoil fold and one carbohydrate-binding site. In order to study the mode of CNL action, a non-sugar-binding mutant and non-dimerizing monovalent mutants that retain carbohydrate-binding activity were prepared. rCNL and the mutants were examined for their biological activities against Jurkat human leukemic T cells and the hypersensitive nematode Caenorhabditis elegans mutant strain pmk-1. rCNL was toxic against both, while the mutants were inactive. Thus, the bivalent carbohydrate-binding property of homodimeric CNL is essential for its activity, providing one of the rare pieces of evidence that certain activities of lectins are associated with their multivalency.
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===Clitocybe nebularis ricin B-like lectin (CNL) in complex with N,N'-diacetyllactosediamine===
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Bivalent carbohydrate binding is required for biological activity of CNL, the LacdiNAc (GalNAcbeta1-4GlcNAc)-specific lectin from basidiomycete Clitocybe nebularis.,Pohleven J, Renko M, Magister S, Smith DF, Kuenzler M, Strukelj B, Turk D, Kos J, Sabotic J J Biol Chem. 2012 Feb 1. PMID:22298779<ref>PMID:22298779</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_22298779}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3nbe" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22298779 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22298779}}
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==About this Structure==
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[[3nbe]] is a 2 chain structure of [[Ricin]] with sequence from [http://en.wikipedia.org/wiki/Clitocybe_nebularis Clitocybe nebularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBE OCA].
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==See Also==
==See Also==
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*[[Ricin]]
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*[[Ricin 3D structures|Ricin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022298779</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Clitocybe nebularis]]
[[Category: Clitocybe nebularis]]
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[[Category: Kos, J.]]
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[[Category: Large Structures]]
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[[Category: Pohleven, J.]]
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[[Category: Kos J]]
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[[Category: Renko, M.]]
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[[Category: Pohleven J]]
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[[Category: Sabotic, J.]]
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[[Category: Renko M]]
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[[Category: Turk, D.]]
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[[Category: Sabotic J]]
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[[Category: Clitocybe nebularis ricin b-like lectin]]
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[[Category: Turk D]]
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[[Category: N'-diacetyllactosediamine]]
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[[Category: Sugar binding protein]]
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Current revision

Clitocybe nebularis ricin B-like lectin (CNL) in complex with N,N'-diacetyllactosediamine

PDB ID 3nbe

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